Merck
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D5030

Sigma-Aldrich

杜氏改良 Eagle 培养基-低葡萄糖

Without glucose, L-glutamine, phenol red, sodium pyruvate and sodium bicarbonate, powder, suitable for cell culture

Synonym(s):
DME, DMEM
NACRES:
NA.75

质量水平

形式

powder

technique(s)

cell culture | mammalian: suitable

组分

phenol red: no
NaHCO3: no
L-glutamine: no
HEPES: no
glucose: no
sodium pyruvate: no

运输

ambient

储存温度

2-8°C

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51441C51435C56436C
SAFC

SAFC

51441C

培养基

form

powder

form

liquid

form

liquid

form

powder

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable, cell culture | stem cell: suitable

technique(s)

cell culture | mammalian: suitable

components

phenol red: no
NaHCO3: no
L-glutamine: no
HEPES: no
glucose: no
sodium pyruvate: no

components

glucose: high
phenol red: yes
sodium pyruvate: no
L-glutamine: yes

components

L-glutamine: no
glucose: high
phenol red: yes
sodium pyruvate: yes

components

phenol red: no
L-glutamine: yes
glucose: high
sodium pyruvate: no

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

500

Quality Level

500

Quality Level

500

Quality Level

500

一般描述

最基本的配方。这个配方适用于希望从DMEM培养基的基础成分开始研究的实验人员,可以根据应用灵活优化配方。
杜氏改良Eagle培养基(DMEM)是对Eagle基础培养基 (BME)的改良,含有四倍浓度的氨基酸和维生素。它包括额外的补充部分。据报告首个DME配方用于培养含有1,000 mg/L葡萄糖的胚胎小鼠细胞。用4,500 mg/L葡萄糖修饰培养基对培养某些类型的细胞效果最佳。

应用

杜氏改良Eagle′培养基已用于:
  • 培养分离的指短屈肌(FDB)纤维,以测量外源脂肪酸(FA)的利用率,作为耗氧率(OCR)测量的一部分
  • 培养离体胫骨前肌纤维以测定乳酸含量
  • 培养用于成骨分化的人骨髓间充质干细胞,制备培养人肝细胞癌HepG2细胞的低糖培养基

数量

按照每升培养基8.3克粉末配制。

重悬

补充1.0g/L葡萄糖,0.584g/L L-谷氨酰胺,3.7g/L碳酸氢钠。

象形图

Exclamation mark

警示用语:

Warning

危险声明

危险分类

Eye Irrit. 2

储存分类代码

13 - Non Combustible Solids

WGK

WGK 1

闪点(F)

Not applicable

闪点(C)

Not applicable


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