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D5030

Sigma-Aldrich

杜氏改良 Eagle 培养基-低葡萄糖

Without glucose, L-glutamine, phenol red, sodium pyruvate and sodium bicarbonate, powder, suitable for cell culture

别名:

DME, DMEM

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About This Item

分類程式碼代碼:
12352207
NACRES:
NA.75

品質等級

500

形狀

powder

技術

cell culture | mammalian: suitable

成分

phenol red: no
NaHCO3: no
L-glutamine: no
HEPES: no
glucose: no
sodium pyruvate: no

運輸包裝

ambient

儲存溫度

2-8°C

相关类别

一般說明

最基本的配方。这个配方适用于希望从DMEM培养基的基础成分开始研究的实验人员，可以根据应用灵活优化配方。
杜氏改良Eagle培养基（DMEM）是对Eagle基础培养基 (BME)的改良，含有四倍浓度的氨基酸和维生素。它包括额外的补充部分。据报告首个DME配方用于培养含有1,000 mg/L葡萄糖的胚胎小鼠细胞。用4,500 mg/L葡萄糖修饰培养基对培养某些类型的细胞效果最佳。

應用

杜氏改良Eagle′培养基已用于：
  • 培养分离的指短屈肌（FDB）纤维，以测量外源脂肪酸（FA）的利用率，作为耗氧率（OCR）测量的一部分
  • 培养离体胫骨前肌纤维以测定乳酸含量
  • 培养用于成骨分化的人骨髓间充质干细胞，制备培养人肝细胞癌HepG2细胞的低糖培养基

數量

按照每升培养基8.3克粉末配制。

重構

补充1.0g/L葡萄糖，0.584g/L L-谷氨酰胺，3.7g/L碳酸氢钠。

其他說明

2024年CiteAb奖——成功的帕金森研究供应商(2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research)

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補充物

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说明
价格

象形圖

Exclamation mark
GHS07

訊號詞

Warning

危險聲明

H319

危險分類

Eye Irrit. 2

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

分析证书(COA)

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粉末培养基制备说明

粉末培养基及盐混合物极具吸湿性，应注意防潮。

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