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D5652

Sigma-Aldrich

杜氏磷酸盐缓冲盐水

Modified, without calcium chloride and magnesium chloride, powder, suitable for cell culture

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别名:
DPBS
MDL编号:
MFCD00131855
eCl@ss:
32129211

质量水平

500

形式

powder

technique(s)

cell culture | mammalian: suitable

pH值（酸碱度）

7.2-7.8

溶解性

water: 9.6 g/L, clear

储存温度

2-8°C

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此商品
D1283D4031D5773
Dulbecco’s Phosphate Buffered Saline Modified, without calcium chloride and magnesium chloride, powder, suitable for cell culture

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D5652

杜氏磷酸盐缓冲盐水

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D1283

杜氏磷酸盐缓冲盐水

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D4031

杜氏磷酸盐缓冲盐水

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D5773

杜氏磷酸盐缓冲盐水

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

pH

7.2-7.8

pH

-

pH

-

pH

-

solubility

water: 9.6 g/L, clear

solubility

-

solubility

-

solubility

-

storage temp.

2-8°C

storage temp.

-

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

500

Quality Level

-

Quality Level

-

Quality Level

500

一般描述

杜氏(Dulbecco′s)磷酸盐缓冲液(DPBS)是在组织培养中广泛使用的平衡盐溶液(BSS)。

应用

在制备和连续转移过程中，可使用D-PBS清洗细胞。
杜氏磷酸盐缓冲液已用于：
  • 作为模拟生理溶液成分,体外研究Fe–20Mn–1.2C合金的降解行为
  • 溶解朝鲜当归(Angelica gigas)粉末进行微囊化
  • 在细胞培养物处理、愈伤试验、transwell试验和凋亡试验中用于清洗细胞
  • 洗涤羊膜用于细胞分析

生化/生理作用

杜氏磷酸盐缓冲液可维持细胞渗透压平衡，作为灌洗、运输和稀释用液。它提供正常细胞代谢必需的水和大量无机离子。混入葡萄糖等糖后，这种缓冲盐可提供细胞代谢所需的主要能量。它的缓冲能力可使培养基维持在生理pH范围内。

数量

按照每升培养基9.6克粉末配制。

其他说明

该D-PBS配方中没有Ca或Mg。也可提供加入Ca和Mg的配方

储存分类代码

11 - Combustible Solids

WGK

WGK 1

闪点(F)

Not applicable

闪点(C)

Not applicable

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Journal of colloid and interface science, 506, 393-402 (2017-07-28)
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