Merck
All Photos(5)

D8417

Sigma-Aldrich

4′,6-二脒基-2-苯基吲哚 二盐酸盐

powder, BioReagent, suitable for cell culture, ≥98% (HPLC and TLC), suitable for fluorescence

Synonym(s):
2-(4-氨基苯基)-6-吲哚甲酰胺 二盐酸盐, DAPI 二盐酸盐
Empirical Formula (Hill Notation):
C16H15N5 · 2HCl
CAS Number:
Molecular Weight:
350.25
Beilstein:
4894417
EC 号:
MDL编号:
PubChem化学物质编号:
NACRES:
NA.75

产品线

BioReagent

质量水平

200

测定

≥98% (HPLC and TLC)

形式

powder

technique(s)

cell culture | mammalian: suitable

溶解性

H2O: 20 mg/mL (Heat or sonication may be required. Solutions stored in the dark at room temperature or 4 °C should be stable for 2 to 3 weeks.)
PBS: insoluble

ε (extinction coefficient)

30 at 263 nm in H2O

荧光

λex 340 nm; λem 488 nm (nur DAPI)
λex 364 nm; λem 454 nm (DAPI-DNA-Komplex)

适用性

suitable for fluorescence

储存温度

2-8°C

SMILES string

Cl.Cl.NC(=N)c1ccc(cc1)-c2cc3ccc(cc3[nH]2)C(N)=N

InChI

1S/C16H15N5.2ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;;/h1-8,21H,(H3,17,18)(H3,19,20);2*1H

InChI key

FPNZBYLXNYPRLR-UHFFFAOYSA-N

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一般描述

4′,6-二脒基-2-苯基吲哚二盐酸盐是一种用于DNA染色的荧光染料。[1]它与DNA中富含 dAT(脱氧腺嘌呤-胸腺嘧啶)区域强烈地相互作用。[2]

应用

4′,6-二脒基-2-苯基吲哚二盐酸盐应用于免疫细胞化学和细胞化学中各种细胞的染色,[3]人类皮下脂肪组织的荧光成像、[4]免疫荧光胚胎切片。[5]
在琼脂糖凝胶中染色DNA,DAPI比溴化乙锭敏感几倍。它可用于DNA的光足迹图谱,通过对双链复合体的特殊可视化检测印迹应用中的退火探针,并使用流式细胞分析术研究DNA中的变化并分析凋亡过程中的DNA含量。DAPI染色也是一种敏感和特异的支原体检测方法。

包装

1, 5, 10 mg in glass bottle

生化/生理作用

DNA的细胞渗透性荧光小沟结合探针。与双链DNA的小沟(富含AT的DNA优先)结合,形成稳定的复合物,其发出的荧光比单独的DAPI高约20倍。

注意

避光保存。

象形图

Exclamation mark

警示用语:

Warning

危险声明

危险分类

Skin Irrit. 2 - Skin Sens. 1A - STOT SE 3

靶器官

Respiratory system

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(F)

Not applicable

闪点(C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

Certificate of Analysis

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Certificate of Origin

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