Merck

D8418

Sigma-Aldrich

二甲基亚砜

for molecular biology

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别名:
分子生物学级DMSO, 甲基亚砜, 甲基硫酰基甲烷, DMSO
线性分子式:
(CH3)2SO
CAS号:
分子量:
78.13
Beilstein:
506008
EC 号:
MDL编号:
PubChem化学物质编号:
NACRES:
NA.52

等级

for molecular biology

质量水平

蒸汽密度

2.7 (vs air)

蒸汽压

0.42 mmHg ( 20 °C)

产品线

BioReagent

检测方案

≥99.9%

形式

liquid

自燃温度

573 °F

保质期

Recommended retest period - 2 years

expl. lim.

42 %, 63 °F

technique(s)

DNA sequencing: suitable
PCR: suitable
transfection: suitable

杂质

≤0.001 meq/g Titratable acid
≤0.1% water (Karl Fischer)

颜色

colorless

折射率

n20/D 1.479 (lit.)

bp

189 °C (lit.)

mp

16-19 °C (lit.)

溶解性

H2O: miscible (completely)

密度

1.10 g/mL (lit.)

吸光度

passes test

适用性

suitable for molecular biology

异质活性

DNase and RNase, none detected

储存温度

room temp

SMILES string

CS(C)=O

InChI

1S/C2H6OS/c1-4(2)3/h1-2H3

InChI key

IAZDPXIOMUYVGZ-UHFFFAOYSA-N

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D2438D4540D9170
Dimethyl sulfoxide for molecular biology

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D8418

二甲基亚砜

Dimethyl sulfoxide sterile-filtered, BioPerformance Certified, meets EP, USP testing specifications, suitable for hybridoma

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Dimethyl sulfoxide ≥99.5% (GC), suitable for plant cell culture

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D4540

二甲基亚砜

Dimethyl sulfoxide PCR Reagent

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vapor density

2.7 (vs air)

vapor density

2.7 (vs air)

vapor density

2.7 (vs air)

vapor density

2.7 (vs air)

vapor pressure

0.42 mmHg ( 20 °C)

vapor pressure

0.42 mmHg ( 20 °C)

vapor pressure

0.42 mmHg ( 20 °C)

vapor pressure

0.42 mmHg ( 20 °C)

assay

≥99.9%

assay

-

assay

≥99.5% (GC)

assay

≥99.5%

form

liquid

form

liquid

form

liquid

form

liquid

autoignition temp.

573 °F

autoignition temp.

573 °F

autoignition temp.

573 °F

autoignition temp.

573 °F

一般描述

特点及优势:

  • 适于分子应用
  • 无核酸酶DMSO
  • 不含DNase、RNase、蛋白酶和磷酸酶
  • 分子生物学级DMSO

二甲基亚砜(DMSO)是一种对有机和无机化学品具有出色溶剂性质的高极性有机试剂。
该产品为分子生物学级,适用于分子生物学应用,对其中核酸酶和蛋白酶的存在也进行了分析。

应用

DMSO(二甲基亚砜)是一种在有机化学和分子生物学中具有诸多应用的高度极性、非质子有机溶剂。DMSO通常用于

  • 聚合酶链式反应 (PCR)
  • cDNA文库扩增
  • DNA测序
  • poly(A)+ RNA筛选的上柱缓冲液
  • 感受态大肠杆菌转化缓冲液
  • 转染方案。

二甲基亚砜已用于-

  • 用作芯片点样应用中的寡核苷酸溶剂[1]
  • 用作miRNA芯片分析和实时荧光定量PCR (qRT-PCR)中的溶剂
  • 在MTT(3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四唑溴化物)测定的细胞裂解液
  • 制备MMP(线粒体膜电位)细胞荧光评估过程所用的储备溶液
  • 用作储存人类和动物细胞系和噬菌体λ的冷冻保护剂。
制备无菌过滤DMSO溶液时,建议使用PTFE或尼龙膜。不建议使用醋酸纤维素膜。

特点和优势

该产品不含DNase、RNase、磷酸酶和蛋白酶。

注意

在室温下容易过冷并缓慢重融。 固化产品可以通过加热至室温来重新液化,而不会损害产品。

储存分类代码

10 - Combustible liquids

WGK

WGK 1

闪点(°F)

188.6 °F - closed cup

闪点(°C)

87 °C - closed cup

个人防护装备

Eyeshields, Gloves, type ABEK (EN14387) respirator filter


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