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D9154

Sigma-Aldrich

邻联茴香胺 二盐酸盐

tablet

别名:

3,3'-二甲氧基联苯胺 二盐酸盐, 固蓝 B

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About This Item

经验公式（希尔记法）:
C14H16N2O2 · 2HCl
CAS号:
20325-40-0
分子量:
317.21
Beilstein:
3917996
EC號碼:
243-737-5
MDL號碼:
MFCD00012488
分類程式碼代碼:
12352204
PubChem物質ID:
24894263
NACRES:
NA.83

产品名称

邻联茴香胺 二盐酸盐, tablet, 10 mg substrate per tablet

形狀

tablet

溶解度

water: 1 tablet/10 mL, clear, colorless

儲存溫度

2-8°C

SMILES 字串

Cl.Cl.COc1cc(ccc1N)-c2ccc(N)c(OC)c2

InChI

1S/C14H16N2O2.2ClH/c1-17-13-7-9(3-5-11(13)15)10-4-6-12(16)14(8-10)18-2;;/h3-8H,15-16H2,1-2H3;2*1H

InChI 密鑰

UXTIAFYTYOEQHV-UHFFFAOYSA-N

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相关类别

特異性

邻联茴香胺（3,3′-dimethoxybenzidine）是一种适合在 ELISA 步骤中使用的过氧化物酶底物。该底物产生可溶性终产物，颜色为黄橙色，可在 405 nm 处用分光光度法读取。可用 5 MHCl 终止反应。

警告

可能致癌。

重構

Dissolve one tablet in 60 ml of 50 mM phosphate-citrate buffer, pH 5.0. Add 12 μl of fresh 30% hydrogen peroxide immediately before to use.

訊號詞

Danger

危險聲明

H302,H314,H350

危險分類

Acute Tox. 4 Oral - Carc. 1B - Eye Dam. 1 - Skin Corr. 1

儲存類別代碼

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves, type P3 (EN 143) respirator cartridges

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
SLCJ7840
SLCD6994
SLBX3894
SLBR5244V
SLBN0046V

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M Kaszuba et al.
Biochimica et biophysica acta, 1419(2), 221-228 (1999-07-17)
Reactive cationic and anionic liposomes have been prepared from mixtures of dimyristoylphosphatidylcholine (DMPC) and cholesterol incorporating dimethyldioctadecylammonium bromide and DMPC incorporating phosphatidylinositol, respectively. The liposomes were prepared by the vesicle extrusion technique and had the enzymes glucose oxidase (GO) encapsulated
Frédéric Frottin et al.
Molecular & cellular proteomics : MCP, 5(12), 2336-2349 (2006-09-12)
Methionine aminopeptidase (MAP) is a ubiquitous, essential enzyme involved in protein N-terminal methionine excision. According to the generally accepted cleavage rules for MAP, this enzyme cleaves all proteins with small side chains on the residue in the second position (P1')
Wei Shen et al.
Biosensors & bioelectronics, 44, 171-176 (2013-02-22)
Herein we report a label-free microRNA (miRNA) biosensor in which the formation of a thin insulating film is used to amplify the analytical signal. Briefly, the biosensor is made of an oligonucleotide-coated gold electrode. After hybridizing with a target miRNA
Ozcan Erel
Clinical biochemistry, 38(12), 1103-1111 (2005-10-11)
To develop a new, colorimetric and automated method for measuring total oxidation status (TOS). The assay is based on the oxidation of ferrous ion to ferric ion in the presence of various oxidant species in acidic medium and the measurement
Iain S MacPherson et al.
Protein engineering, design & selection : PEDS, 23(3), 137-145 (2010-01-20)
Directed evolution methods were developed for Cu-containing nitrite reductase (NiR) from Alcaligenes faecalis S-6. The PCR cloning strategy allows for the efficient production of libraries of 100 000 clones by a modification of a megaprimer-based whole-plasmid synthesis reaction. The high-throughput
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实验方案

Enzymatic Assay of Diamine Oxidase (E.C. No. 1.4.3.22)

To standardize a procedure for the enzymatic assay of Diamine Oxidase.

Enzymatic Assay: Glucose Oxidase

Glucose oxidase activity measured via continuous spectrophotometric assay at 500 nm, indicating glucose oxidation rate.

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Enzymatic activity assay for cholesterol oxidase, excluding specific product numbers, providing guidelines for accurate cholesterol oxidase assays.

胆固醇氧化酶的酶学测定

本操作流程使用过氧化物酶和邻联二茴香胺在500nm处进行胆固醇氧化酶活性的测定。胆固醇氧化酶是一种以氧为受体而作用于供体的CH-OH基团的酶。

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