D9542
DAPI
for nucleic acid staining
Recommended Products
等级
for molecular biology
检测方案
≥98% (HPLC)
形式
powder
technique(s)
transfection: suitable
溶解性
H2O: 20 mg/mL (heat or sonication may be required. Solutions stored in the dark at room temperature or 4 °C should be stable for 2 to 3 weeks.)
PBS: insoluble
ε (消光系数)
30 at 263 nm in H2O at 1 mM
荧光
λex 340 nm; λem 488 nm (nur DAPI)
λex 364 nm; λem 454 nm (DAPI-DNA-Komplex)
适用性
suitable for fluorescence
储存温度
2-8°C
SMILES string
Cl.Cl.NC(=N)c1ccc(cc1)-c2cc3ccc(cc3[nH]2)C(N)=N
InChI
1S/C16H15N5.2ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;;/h1-8,21H,(H3,17,18)(H3,19,20);2*1H
Inchi Key
FPNZBYLXNYPRLR-UHFFFAOYSA-N
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This Item | MBD0015 | MBD0020 | 5.08741 |
|---|---|---|---|
| grade for molecular biology | grade - | grade - | grade - |
| assay ≥98% (HPLC) | assay - | assay - | assay ≥97% (HPLC) |
| form powder | form liquid | form liquid | form liquid |
| technique(s) transfection: suitable | technique(s) transfection: suitable | technique(s) DNA extraction: suitable, DNA sequencing: suitable, PCR: suitable | technique(s) - |
| solubility H2O: 20 mg/mL (heat or sonication may be required. Solutions stored in the dark at room temperature or 4 °C should be stable for 2 to 3 weeks.), PBS: insoluble | solubility - | solubility - | solubility - |
| ε (extinction coefficient) 30 at 263 nm in H2O at 1 mM | ε (extinction coefficient) - | ε (extinction coefficient) - | ε (extinction coefficient) - |
一般描述
应用
- 琼脂糖凝胶中的DNA染色
- 分析凋亡过程中DNA的变化
- 支原体检测
- DNA的光足迹图谱
- 细胞的免疫荧光染色
DAPI已用于：
- 微生物污染的快速监测
- 染色体条带技术
- 凋亡细胞的检测
- 在荧光显微镜中追踪DisA（DNA完整性扫描蛋白）在枯草芽孢杆菌DNA上的移动
- 对成熟的花粉粒进行染色（0.5 mg/ml）
生化/生理作用
注意
警示用语：
Warning
危险分类
Skin Irrit. 2 - Skin Sens. 1A - STOT SE 3
靶器官
Respiratory system
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(F)
Not applicable
闪点(C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
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