Merck
All Photos(6)

D9542

Sigma-Aldrich

DAPI

for nucleic acid staining

Synonym(s):
4′,6-二脒基-2-苯基吲哚 二盐酸盐, 2-(4-氨基苯基)-6-吲哚甲酰胺 二盐酸盐, DAPI 二盐酸盐
Empirical Formula (Hill Notation):
C16H15N5 · 2HCl
CAS Number:
Molecular Weight:
350.25
Beilstein:
4894417
EC 号:
MDL编号:
PubChem化学物质编号:
NACRES:
NA.52

等级

for molecular biology

检测方案

≥98% (HPLC)

形式

powder

technique(s)

transfection: suitable

溶解性

H2O: 20 mg/mL (heat or sonication may be required. Solutions stored in the dark at room temperature or 4 °C should be stable for 2 to 3 weeks.)
PBS: insoluble

ε (消光系数)

30 at 263 nm in H2O at 1 mM

荧光

λex 340 nm; λem 488 nm (nur DAPI)
λex 364 nm; λem 454 nm (DAPI-DNA-Komplex)

适用性

suitable for fluorescence

储存温度

2-8°C

SMILES string

Cl.Cl.NC(=N)c1ccc(cc1)-c2cc3ccc(cc3[nH]2)C(N)=N

InChI

1S/C16H15N5.2ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;;/h1-8,21H,(H3,17,18)(H3,19,20);2*1H

Inchi Key

FPNZBYLXNYPRLR-UHFFFAOYSA-N

Looking for similar products? Visit Product Comparison Guide

Related Categories

Compare Similar Items

View Full Comparison

1 of 4

This Item
MBD0015MBD00205.08741
DAPI for nucleic acid staining

Sigma-Aldrich

D9542

DAPI

DAPI ready made solution For Nuclear counterstain in immunoflourecence microscopy, High Content Screening (HCS), Chromosome staining and flow cytometry (FACS)., 1 mg/mL

Sigma-Aldrich

MBD0015

DAPI成品溶液

DAPI Stain

Millipore

5.08741

DAPI Stain

grade

for molecular biology

grade

-

grade

-

grade

-

assay

≥98% (HPLC)

assay

-

assay

-

assay

≥97% (HPLC)

form

powder

form

liquid

form

liquid

form

liquid

technique(s)

transfection: suitable

technique(s)

transfection: suitable

technique(s)

DNA extraction: suitable, DNA sequencing: suitable, PCR: suitable

technique(s)

-

solubility

H2O: 20 mg/mL (heat or sonication may be required. Solutions stored in the dark at room temperature or 4 °C should be stable for 2 to 3 weeks.), PBS: insoluble

solubility

-

solubility

-

solubility

-

ε (extinction coefficient)

30 at 263 nm in H2O at 1 mM

ε (extinction coefficient)

-

ε (extinction coefficient)

-

ε (extinction coefficient)

-

一般描述

DAPI(4′,6-二脒基-2-苯基吲哚二盐酸盐)是一种可与DNA结合的细胞可通透荧光染料。

应用

在琼脂糖凝胶中染色DNA,DAPI比溴化乙锭敏感几倍。它可用于DNA的光足迹图谱,通过对双链复合体的特殊可视化检测印迹应用中的退火探针,并使用流式细胞分析术研究DNA中的变化并分析凋亡过程中的DNA含量。DAPI染色也是一种敏感和特异的支原体检测方法。
适用于
  • 琼脂糖凝胶中的DNA染色
  • 分析凋亡过程中DNA的变化
  • 支原体检测
  • DNA的光足迹图谱
  • 细胞的免疫荧光染色

DAPI已用于:
  • 微生物污染的快速监测
  • 染色体条带技术
  • 凋亡细胞的检测
  • 在荧光显微镜中追踪DisA(DNA完整性扫描蛋白)在枯草芽孢杆菌DNA上的移动
  • 对成熟的花粉粒进行染色(0.5 mg/ml)

生化/生理作用

DNA的细胞渗透性荧光小沟结合探针。与双链DNA的小沟(富含AT的DNA优先)结合,形成稳定的复合物,其发出的荧光比单独的DAPI高约20倍。

注意

避光保存。

象形图

Exclamation mark

警示用语:

Warning

危险声明

危险分类

Skin Irrit. 2 - Skin Sens. 1A - STOT SE 3

靶器官

Respiratory system

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(F)

Not applicable

闪点(C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (Example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service