Merck
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D9909

Sigma-Aldrich

Dextran Sucrase from Leuconostoc mesenteroides

lyophilized powder, ≥100 units/mg protein

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CAS号:
9032-14-8
EC 号:
2.4.1.5 (BRENDA, IUBMB)
MDL编号:
MFCD00130936
NACRES:
NA.54

生物来源

bacterial (Leuconostoc mesenteroides)

质量水平

200

形式

lyophilized powder

specific activity

≥100 units/mg protein

组成

Protein, ~15% Lowry

溶解性

H2O: soluble 0.9-1.1 mg/mL, clear to slightly hazy, colorless to light yellow

储存温度

−20°C

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此商品
P5538D8802M9532
Dextran Sucrase from Leuconostoc mesenteroides lyophilized powder, ≥100 units/mg protein

Sigma-Aldrich

D9909

Dextran Sucrase from Leuconostoc mesenteroides

Sigma-Aldrich

Sigma-Aldrich

P5538

磷酸葡糖异构酶 来源于嗜热脂肪芽胞杆菌

Sigma-Aldrich

Sigma-Aldrich

D8802

葡聚糖 溶液 来源于肠系膜明串珠菌

Mannitol Dehydrogenase from Leuconostoc mesenteroides lyophilized powder, ≥60 units/mg protein

Sigma-Aldrich

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甘露醇脱氢酶 来源于肠系膜明串珠菌

form

lyophilized powder

form

lyophilized powder

form

liquid

form

lyophilized powder

specific activity

≥100 units/mg protein

specific activity

300-1,000 units/mg protein

specific activity

-

specific activity

≥60 units/mg protein

solubility

H2O: soluble 0.9-1.1 mg/mL, clear to slightly hazy, colorless to light yellow

solubility

-

solubility

-

solubility

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

一般描述

Dextran Sucrase from Leuconostoc mesenteroides belongs to glycoside hydrolase family 70 (GH70). It functions through a retaining mechanism and uses two catalytic acidic residues. Dextran sucrase has a dextran binding site in the C-terminal domain.

应用

Dextran Sucrase from Leuconostoc mesenteroides has been used:
  • in immobilization on shirasu porous membrane (SPG) for dextran production
  • in the enzymatic synthesis of dextran nanoparticles at various pH range
  • in the immobilization with hydroxyapatite for dextran production

Dextran sucrase from Leuconostoc mesenteroides has been used in a study to investigate the functional and structural characterization of α-(1→2) branching sucrase derived from DSR-E glucansucrase. Dextran sucrase from Leuconostoc mesenteroides has also been used in a study to investigate the bioengineering of Leuconostoc mesenteroides glucansucrases.
The enzyme from Sigma has been used to prepare immobilized sphere for the production of dextran from sucrose.

生化/生理作用

Dextransucrases are glucansucrases that are able to produce dextran, a glucose polymer linked mainly through α1-6 bonds. However, α1-3, α1-6, α1-4 and α1-2 bonds are also found, in both the main chain and the branching linkages. The peptide has approximately 1600 amino acids. The aspartic acid in position 551 is essential for catalytic activity, while glutamic acid 589 and aspartic acid 662 complement the catalytic triad. The activity of dextransucrase is decreased by EDTA, and is restored by the addition of calcium ions. Zinc, cadmium, lead, mercury and copper ions are inhibitory to various degrees.

质量

色谱纯化产品

单位定义

One unit will liberate 1.0 μmole of fructose per min at 37 °C, pH 5.4.

外形

Lyophilized powder containing dextran, MES buffer salts and CaCl2

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(F)

Not applicable

闪点(C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

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Ah-Rum Yi et al.
Journal of microbiology and biotechnology, 19(8), 829-835 (2009-09-08)
A dextransucrase (LcDS) gene from Leuconostoc citreum HJ-P4 has been amplified and cloned in E. coli. The LcDS gene consists of 4,431 nucleotides encoding 1,477 amino acid residues sharing 63-98% of amino acid sequence identities with other known dextransucrases from
Bioengineering of Leuconostoc mesenteroides glucansucrases that gives selected bond formation for glucan synthesis and/or acceptor-product synt
Kang, H., et al.
RNA, 59, 4148-4155 (2011)
Marie-Sophie Bounaix et al.
FEMS microbiology letters, 311(1), 18-26 (2010-08-21)
The study of exopolysaccharide production by heterofermentative sourdough lactic acid bacteria has shown that Weissella strains isolated from sourdoughs produce linear dextrans containing α-(1→6) glucose residues with few α-(1→3) linkages from sucrose. In this study, several dextran-producing strains, Weissella cibaria
Hidetaka Kawakita et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 877(3), 347-350 (2009-01-03)
Dextransucrase forms a complex with dextran during an enzymatic reaction with sucrose. Using its enzymatic character, we performed a continuous and dynamic rejection of colloidal particles by generating dextran with dextransucrase immobilized in an inorganic porous membrane. Inorganic membranes having
Hongbin Zhang et al.
Sheng wu gong cheng xue bao = Chinese journal of biotechnology, 25(12), 2022-2028 (2010-04-01)
We optimized the medium for recombinant dextransucrase expression in engineering strain Escherichia coli BL21 (DE3)/pET28-dexYG by an Orthogonal experiment. After the medium had been decided, we studied the effect of temperature, sucrose concentration and pH value on the yield. The
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