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E1014

Millipore

Benzonase®核酸酶

≥250 units/μL, ≥90% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous glycerol solution

别名:
内切核酸酶 来源于粘质沙雷氏菌
CAS号:
MDL编号:
NACRES:
NA.54

质量水平

200

重组

expressed in E. coli

测定

≥90% (SDS-PAGE)

形式

buffered aqueous glycerol solution

分子量

30 kDa

浓度

≥250 units/μL

异质活性

protease, essentially free

运输

wet ice

储存温度

−20°C

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应用

Benzonase核酸酶,或来自粘质沙雷氏菌的内切核酸酶,可用于降解所有形式的DNA及RNA,并同时不带有蛋白水解活性。Benzonase核酸酶还可在微量热实验中用于制备蛋白质。
来自sigma的该酶已用于在嵌合细胞混合物的制备中限制细胞的成团。它还用于通过消化DNA并释放与DNA密切相关的核蛋白来进行核提取物的制备。
用于去除蛋白样品中的核酸。

生化/生理作用

Benzonase®是一种来自粘质沙雷氏菌的基因工程改造内切核酸酶。该蛋白质是30kDa亚基的二聚体,带有两个必需的二硫键。该内切核酸酶可攻击并降解所有形式的DNA和RNA(单链的、双链的、线性的和环化的),并可在一系列宽泛的操作条件下具有高效性。最适的酶活pH为8.0-9.2。它可将核酸完全消化为长度为3至5个碱基的5′-单磷酸末端寡核苷酸。这对于从重组蛋白中去除核酸以及需要完全消化核酸的应用是最为理想的选择。它还可降低蛋白提取物的粘度并防止细胞成团。对蛋白样品的预处理可通过去除任何结合的核酸而提高它在2D凝胶电泳中的分辨率。
消化天然或热变性的DNA和RNA。

单位定义

在pH 8.0,37 °C条件下,一个单位的酶在30分钟内可将超声的鲑鱼精子DNA消化成等同于1.0 ΔA260的酸可溶寡核苷酸(2.625 ml反应体积)。

外形

在含有20 mM Tris HCl, pH 8.0, 2 mM MgCl2及20 mM NaCl的50%甘油中的溶液。

法律信息

Benzonase®核酸酶由德国达姆施塔特默克公司和/或其附属公司提供。
Benzonase is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

闪点(F)

Not applicable

闪点(C)

Not applicable

个人防护装备

Eyeshields, Gloves

分析证书

原产地证书 (CofO)

Richik Nilay Mukherjee et al.
Molecular biology of the cell, 30(18), 2349-2357 (2019-07-19)
Endoplasmic reticulum (ER) tubules and sheets conventionally correspond to smooth and rough ER, respectively. The ratio of ER tubules-to-sheets varies in different cell types and changes in response to cellular conditions, potentially impacting the functional output of the ER. To...
P Friedhoff et al.
Protein expression and purification, 5(1), 37-43 (1994-02-01)
Overproduction of the extracellular Serratia marcescens nuclease in Escherichia coli results in aggregation and sequestration of a large amount of the protein in inclusion bodies. Only a relatively small amount is secreted into the medium from which it can be...
Jos J M Drabbels et al.
Blood, 118(19), e149-e155 (2011-09-21)
Microchimerism is defined by the presence of low levels of nonhost cells in a person. We developed a reliable method for separating viable microchimeric cells from the host environment. For flow cytometric cell sorting, HLA antigens were targeted with human...
Miles C Scotcher et al.
PloS one, 4(3), e4924-e4924 (2009-03-18)
Botulism, an often fatal neuroparalytic disease, is caused by botulinum neurotoxins (BoNT) which consist of a family of seven serotypes (A-H) produced by the anaerobic bacterium Clostridium botulinum. BoNT, considered the most potent biological toxin known, is a 150 kDa...
T K Ball et al.
Nucleic acids research, 20(19), 4971-4974 (1992-10-11)
The role of the two disulfide bonds found in the Serratia marcescens nuclease were tested by site directed mutagenesis and were found essential for nuclease activity, although slight residual activity remained. The requirement for disulfide bond formation may play a...

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