E1250

Sigma-Aldrich

弹性蛋白酶 来源于猪胰腺

Type I, ≥4.0 units/mg protein

别名:
弹性蛋白酶 来源于猪胰腺, 胰酞酶 E
CAS号:
EC 号:
MDL编号:
NACRES:
NA.54

质量水平

200

类型

Type I

形式

suspension

specific activity

≥4.0 units/mg protein

分子量

25.9 kDa

包含

0.1% thymol

浓度

0.5-15.0 mg/mL in water

异质活性

trypsin ≤50 BAEE units/mg protein

储存温度

2-8°C

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相关类别

应用

来自猪胰腺的弹性蛋白酶已用于研究弹性蛋白酶的仿生亲和配体的设计、合成和评价。来自猪胰腺的弹性蛋白酶也已用于研究胰蛋白水解酶(胰蛋白酶、胰凝乳蛋白酶和弹性蛋白酶)的纯化和部分表征。
来自猪胰腺的弹性蛋白酶已被用于:
  • 诱发腹主动脉瘤(AAA)
  • 研究吲哚胺2-3双加氧酶1(IDO)对小鼠的影响
  • 消化主动脉以分离主动脉平滑肌细胞(SMC)
  • 作为蛋白水解消化的阳性对照

来自Sigma的酶已被用于开发弹性蛋白酶灌注的动物模型。该研究证明了烟草暴露是否可以降低AAA(腹主动脉瘤)发展所需的主动脉损伤阈值。它也被用于从人肺中分离出II型肺细胞。

生化/生理作用

弹性蛋白酶是具有240个氨基酸残基的单一多肽链,其含有四个二硫键。分子量约为25.9kDa。该酶被合成为无活性的酶原形式,即原弹性蛋白酶,其随后通过胰蛋白酶在N-末端的有限蛋白水解而转化为活性形式。它是一种具有广泛特异性的丝氨酸蛋白酶。它可切割在羧基侧具有小疏水性氨基酸(如Ile、Gly、Ala、Ser、Val和Leu)的蛋白质。该酶还可水解酰胺和酯,例如N-苯甲酰基-L-丙氨酸甲酯。其最适pH为8.0-8.5。它不需要任何活化剂,但其活性可被二异丙基氟磷酸盐、苯基甲磺酰氟、α2-巨球蛋白、α1-抗胰蛋白酶、磺酰氟和对二硝基苯基二乙基磷酸盐、以及高盐浓度抑制。它广泛用于组织和细胞解离程序。弹性蛋白酶可有效分离II型肺细胞。
弹性蛋白酶水解弹性蛋白(弹性纤维的特定蛋白质)并消化血红蛋白、酪蛋白和纤维蛋白。

包装

包装规格取决于蛋白含量

单位定义

一个单元将在 25℃ 下,每分钟,pH8.0,水解 1.0 μmole 的 N-琥珀酰-L-Ala-Ala-Ala-对硝基苯胺。

制备说明

2 次结晶

象形图

Exclamation markHealth hazard

警示用语:

Danger

hazcat

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

靶器官

Respiratory system

storage_class_code

11 - Combustible Solids

WGK Germany

WGK 3

闪点(F)

Not applicable

闪点(C)

Not applicable

个人防护装备

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

分析证书

原产地证书 (CofO)

Andreas Giraud et al.
Cardiovascular research, 113(11), 1364-1375 (2017-06-06)
Abdominal aortic aneurysm (AAA), frequently diagnosed in old patients, is characterized by chronic inflammation, vascular cell apoptosis and metalloproteinase-mediated extracellular matrix destruction. Despite improvement in the understanding of the pathophysiology of aortic aneurysm, no pharmacological treatment is yet available to...
Michel P Bergoeing et al.
Journal of vascular surgery, 45(6), 1217-1227 (2007-04-03)
The development of abdominal aortic aneurysms (AAA) is presumed to result from multiple genetic and environmental factors, with exposure to tobacco smoke the single largest known factor predisposing to aneurysm growth. We have attempted to adapt the elastase-perfused animal model...
Júlia Benini Kohler et al.
Biology open, 8(4) (2019-04-12)
Macrophages play a pivotal role in the development of emphysema and depending on the microenvironment stimuli can be polarized into M1- or M2-like macrophage phenotypes. We compared macrophage polarizations in cigarette smoke (CS)- and porcine pancreatic elastase (PPE)-induced emphysema models....
Demetrios Moris et al.
Current vascular pharmacology, 16(2), 168-178 (2017-02-06)
Abdominal aortic aneurysm (AAA) formation is associated with by inflammation and matrix degradation. This study tested the hypothesis that calprotectin, a novel biomarker for inflammation, as well as established biomarkers such as C-reactive protein (CRP) and matrix metalloproteinase- 9 (MMP-9)...
Endothelial MMP-9 drives the inflammatory response in abdominal aortic aneurysm (AAA)
Ramella M, et al.
American Journal of Translational Research, 9(12), 5485-5485 (2017)
实验方案
Enzymatic Assay of Elastase (EC 3.4.21.36), a continuous spectrophotometric rate determination.
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