所有图片(1)

E1250

Sigma-Aldrich

弹性蛋白酶 来源于猪胰腺

Type I, ≥4.0 units/mg protein

别名:
弹性蛋白酶 来源于猪胰腺, 胰酞酶 E
CAS号:
EC 号:
MDL编号:
NACRES:
NA.54

质量水平

200

类型

Type I

形式

suspension

specific activity

≥4.0 units/mg protein

分子量

25.9 kDa

包含

0.1% thymol

浓度

0.5-15.0 mg/mL in water

异质活性

trypsin ≤50 BAEE units/mg protein

储存温度

2-8°C

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相关类别

应用

来自猪胰腺的弹性蛋白酶已用于研究弹性蛋白酶的仿生亲和配体的设计、合成和评价。来自猪胰腺的弹性蛋白酶也已用于研究胰蛋白水解酶(胰蛋白酶、胰凝乳蛋白酶和弹性蛋白酶)的纯化和部分表征。
来自猪胰腺的弹性蛋白酶已被用于:
  • 诱发腹主动脉瘤(AAA)
  • 研究吲哚胺2-3双加氧酶1(IDO)对小鼠的影响
  • 消化主动脉以分离主动脉平滑肌细胞(SMC)
  • 作为蛋白水解消化的阳性对照

来自Sigma的酶已被用于开发弹性蛋白酶灌注的动物模型。该研究证明了烟草暴露是否可以降低AAA(腹主动脉瘤)发展所需的主动脉损伤阈值。它也被用于从人肺中分离出II型肺细胞。

生化/生理作用

弹性蛋白酶是具有240个氨基酸残基的单一多肽链,其含有四个二硫键。分子量约为25.9kDa。该酶被合成为无活性的酶原形式,即原弹性蛋白酶,其随后通过胰蛋白酶在N-末端的有限蛋白水解而转化为活性形式。它是一种具有广泛特异性的丝氨酸蛋白酶。它可切割在羧基侧具有小疏水性氨基酸(如Ile、Gly、Ala、Ser、Val和Leu)的蛋白质。该酶还可水解酰胺和酯,例如N-苯甲酰基-L-丙氨酸甲酯。其最适pH为8.0-8.5。它不需要任何活化剂,但其活性可被二异丙基氟磷酸盐、苯基甲磺酰氟、α2-巨球蛋白、α1-抗胰蛋白酶、磺酰氟和对二硝基苯基二乙基磷酸盐、以及高盐浓度抑制。它广泛用于组织和细胞解离程序。弹性蛋白酶可有效分离II型肺细胞。
弹性蛋白酶水解弹性蛋白(弹性纤维的特定蛋白质)并消化血红蛋白、酪蛋白和纤维蛋白。

包装

包装规格取决于蛋白含量

单位定义

一个单元将在 25℃ 下,每分钟,pH8.0,水解 1.0 μmole 的 N-琥珀酰-L-Ala-Ala-Ala-对硝基苯胺。

制备说明

2 次结晶

象形图

Exclamation markHealth hazard

警示用语:

Danger

危险分类

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

靶器官

Respiratory system

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(F)

Not applicable

闪点(C)

Not applicable

个人防护装备

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

分析证书

原产地证书 (CofO)

Liren Duan et al.
Frontiers in physiology, 11, 866-866 (2020-08-09)
Aim: Smoking is a major risk factor for abdominal aortic aneurysm (AAA). Among the components of smoke, nicotine is known to exert pro-atherosclerotic, prothrombotic, and proangiogenic effects on vascular smooth muscle cells (VSMCs). The current study was designed to investigate
Indoleamine 2 3-dioxygenase knockout limits angiotensin II-induced aneurysm in low density lipoprotein receptor-deficient mice fed with high fat diet
Metghalchi S, et al.
PLoS ONE, 13(3), e0193737-e0193737 (2018)
Takafumi Toyohara et al.
Cell stem cell, 27(1), 147-157 (2020-05-16)
Although susceptibility to cardiovascular disease (CVD) is different for every patient, why some patients with type 2 diabetes mellitus (T2DM) develop CVD while others are protected has not yet been clarified. Using T2DM-patient-derived human induced pluripotent stem cells (hiPSCs), we
Demetrios Moris et al.
Current vascular pharmacology, 16(2), 168-178 (2017-02-06)
Abdominal aortic aneurysm (AAA) formation is associated with by inflammation and matrix degradation. This study tested the hypothesis that calprotectin, a novel biomarker for inflammation, as well as established biomarkers such as C-reactive protein (CRP) and matrix metalloproteinase- 9 (MMP-9)
A C Cunningham et al.
Journal of cell science, 107 ( Pt 2), 443-449 (1994-02-01)
Highly purified populations of alveolar epithelial cells (type II pneumocytes) were isolated from human lung specimens. These cells were characterised histochemically, by demonstrating the presence of intracellular alkaline phosphatase, and morphologically, by electron microscopic demonstration of lamellar bodies and microvilli.

实验方案

Enzymatic Assay of Elastase (EC 3.4.21.36)

Enzymatic Assay of Elastase (EC 3.4.21.36), a continuous spectrophotometric rate determination.

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