Merck

E2156

Sigma-Aldrich

Anti-EGF Receptor antibody, Mouse monoclonal

clone 225, purified from hybridoma cell culture

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别名:
Anti-EGFR, Anti-Epidermal Growth Factor Receptor
MDL编号:
NACRES:
NA.41

生物来源

mouse

质量水平

偶联物

unconjugated

抗体形式

purified immunoglobulin

antibody product type

primary antibodies

克隆

225, monoclonal

形式

buffered aqueous solution

species reactivity

human

浓度

~1.5 mg/mL

technique(s)

immunoprecipitation (IP): 4-8 μg using cell lysate of A431 cells

同位素/亚型

IgG1

UniProt登记号

application(s)

research pathology

运输

dry ice

储存温度

−20°C

target post-translational modification

unmodified

Gene Information

human ... EGFR(1956)

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WH0001956M2E2760SAB4500715
conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

ascites fluid

antibody form

affinity isolated antibody

clone

225, monoclonal

clone

4H2, monoclonal

clone

29.1, monoclonal

clone

polyclonal

form

buffered aqueous solution

form

buffered aqueous solution

form

-

form

buffered aqueous solution

species reactivity

human

species reactivity

human

species reactivity

pig, human, rat, mouse, canine

species reactivity

rat, human, mouse

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一般描述

Monoclonal Anti-EGFR (mouse IgG1 isotype) is derived from the hybridoma 225 produced by the fusion of mouse myeloma cells (NS-1-503 cells) and splenocytes from BALB/c mice immunized with partially purified EGF receptors from A-431 cells. The receptor for epidermal growth factor (EGF) is an integral cell membrane glycoprotein of 170 kDa, which spans the membranes of a wide range of normal and malignant epithelial cells. The EGF receptor has an intracellular domain that exhibits tyrosine kinase activity.

免疫原

partially purified EGF receptors from human A-431 cells.

应用

Monoclonal Anti-EGF Receptor antibody has been used in antibody-nanoparticle conjugation.
Monoclonal Anti-EGF Receptor antibody is suitable for use in immunoprecipitation (4-8 μg using cell lysate of A431 cells).

生化/生理作用

EGFR (epidermal growth factor receptors) protein tyrosine kinase is activated when EGF binds the extracellular binding domain. The first detectable response is the autophosphorylation of the C-terminal tyrosine followed by phosphorylation of other endogenous substrates.
EGFR is a tyrosine kinase receptor that regulates cellular several functions such as growth, blood vessel formation, metastasis and invasion. Alterations in EGFR expression have been associated with a wide range of cancers. Thus, drugs that target EGFR signaling have important therapeutic applications in cancer .

外形

0.2 μm filtered solution in 0.01 M phosphate buffered saline, pH 7.4.

法律信息

This product is for in vitro research use only. It is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for commercial purposes, contact The Regents of the University of California.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存分类代码

10 - Combustible liquids

闪点(°F)

Not applicable

闪点(°C)

Not applicable


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Molecular imaging and quantitative measurement of epidermal growth factor receptor expression in live cancer cells using immunolabeled gold nanoparticles
Crow M J, et al.
American Journal of Respiratory Cell and Molecular Biology, 192(4), 1021-1028 (2009)
Nicole E Willmarth et al.
The Journal of biological chemistry, 281(49), 37728-37737 (2006-10-13)
Amphiregulin (AR) autocrine loops have been associated with several types of cancer. We demonstrate that SUM149 breast cancer cells have a self-sustaining AR autocrine loop. SUM149 cells are epidermal growth factor (EGF)-independent for growth, and they overexpress AR mRNA, AR
Will J Eldridge et al.
Biomedical optics express, 5(8), 2517-2525 (2014-08-20)
We present a fast, wide-field holography system for detecting photothermally excited gold nanospheres with combined quantitative phase imaging. An interferometric photothermal optical lock-in approach (POLI) is shown to improve SNR for detecting nanoparticles (NPs) on multiple substrates, including a monolayer
Molecular imaging of epidermal growth factor receptor in live cells with refractive index sensitivity using dark-field microspectroscopy and immunotargeted nanoparticles
Curry A C, et al.
Journal of Biomedical Optics, 13(1), 014022-014022 (2008)
Melissa C Skala et al.
Methods in molecular biology (Clifton, N.J.), 1026, 85-92 (2013-06-12)
Optical coherence tomography (OCT) is a three-dimensional optical imaging technique that has been successfully implemented in ophthalmology for imaging the human retina, and in studying animal models of disease. OCT can nondestructively visualize structural features in tissue at cellular-level resolution

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