Merck

E2761

Sigma-Aldrich

ExtrAvidin®−FITC

buffered aqueous solution

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MDL编号:
NACRES:
NA.46

偶联物

FITC conjugate

形式

buffered aqueous solution

标记范围

3-6 mol fluorochrome per mol protein

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 20 μg/mL using human tissues
indirect immunofluorescence: 1:200

运输

dry ice

储存温度

−20°C

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E2636E4011A5170
ExtrAvidin®−FITC buffered aqueous solution

Sigma-Aldrich

E2761

ExtrAvidin®−FITC

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

form

-

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 20 μg/mL using human tissues, indirect immunofluorescence: 1:200

technique(s)

dot blot: 1:300,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100 using human tissues sections, indirect ELISA: 1:60,000

technique(s)

flow cytometry: suitable using human peripheral blood lymphocytes with biotinylated antibody, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 5 μg/mL using human tissues

technique(s)

immunoelectrophoresis: suitable, indirect ELISA: 1:100,000

shipped in

dry ice

shipped in

wet ice

shipped in

-

shipped in

dry ice

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

一般描述

亲和素是一种四聚或二聚生物素结合蛋白,在鸟类、爬行动物和两栖动物的输卵管中产生,并沉积在卵白中。它由四个生物素的高亲和力结合位点组成。外膜蛋白由蛋清亲和素制备而成。它是亲和纯化亲和素的改性形式,结合亲和素的高比活性和链霉亲和素的低背景染色。它是一种生物素结合蛋白产生的细菌Streptomyces avidinii。ExtrAvidin已与荧光素异硫氰酸酯(FITC)结合,并用于各种技术。

应用

ExtrAvidin®-FITC适用于以下应用:
  • 免疫组化(福尔马林固定,石蜡包埋切片),浓度为20μg/mL,使用人体组织。
  • 免疫荧光
  • 竞争结合试验

外形

0.01 M磷酸盐缓冲盐水溶液,包含10% (v/v) 0.5 M碳酸盐缓冲液和15 mM叠氮化钠溶液。

制备说明

亲和纯化蛋白

法律信息

ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的人类或动物食用或应用。

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable


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Maria Cristina D Picardo et al.
The Journal of physiology, 591(10), 2687-2703 (2013-03-06)
Breathing in mammals depends on an inspiratory-related rhythm that is generated by glutamatergic neurons in the pre-Bötzinger complex (preBötC) of the lower brainstem. A substantial subset of putative rhythm-generating preBötC neurons derive from a single genetic line that expresses the
D R Hampson et al.
The Journal of biological chemistry, 274(47), 33488-33495 (1999-11-24)
Metabotropic glutamate receptors (mGluRs) are G-protein-coupled glutamate receptors that subserve a number of diverse functions in the central nervous system. The large extracellular amino-terminal domains (ATDs) of mGluRs are homologous to the periplasmic binding proteins in bacteria. In this study
Expression and localization of urokinase-type plasminogen activator receptor in bovine cumulus--oocyte complexes
Garcia DC
Zygote, 24, 230-235 (2016)
J L Tremoleda et al.
European journal of nuclear medicine and molecular imaging, 43(9), 1710-1722 (2016-05-08)
Traumatic spinal cord injury (SCI) is a devastating condition which affects millions of people worldwide causing major disability and substantial socioeconomic burden. There are currently no effective treatments. Modulating the neuroinflammatory (NI) response after SCI has evolved as a major
Xueying Wang et al.
eLife, 3, e03427-e03427 (2014-07-17)
To understand the neural origins of rhythmic behavior one must characterize the central pattern generator circuit and quantify the population size needed to sustain functionality. Breathing-related interneurons of the brainstem pre-Bötzinger complex (preBötC) that putatively comprise the core respiratory rhythm

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