Merck
  • E2886
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E2886

Sigma-Aldrich

ExtrAvidin®-过氧化物酶

buffered aqueous solution

MDL编号:
NACRES:
NA.46

偶联物

peroxidase conjugate

质量水平

形式

buffered aqueous solution

technique(s)

direct ELISA: 1:2,000
dot blot: 1:4,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100

运输

wet ice

储存温度

2-8°C

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This Item
E2636E4011E4142
Sigma-Aldrich

Sigma-Aldrich

E4142

ExtrAvidin®−Cy3

conjugate

peroxidase conjugate

conjugate

alkaline phosphatase conjugate

conjugate

phycoerythrin (R-PE) conjugate

conjugate

CY3 conjugate

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

technique(s)

direct ELISA: 1:2,000, dot blot: 1:4,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100

technique(s)

dot blot: 1:300,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100 using human tissues sections, indirect ELISA: 1:60,000

technique(s)

flow cytometry: suitable using human peripheral blood lymphocytes with biotinylated antibody, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 5 μg/mL using human tissues

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100

shipped in

wet ice

shipped in

wet ice

shipped in

-

shipped in

wet ice

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

特异性

通过免疫电泳发现本品与抗亲和素(目录号 A5170)反应,与抗链霉亲和素(目录号 S6390)不反应。

应用

ExtrAvidin ®-过氧化物酶适用于以下应用:
  • 最低稀释度为 1:4,000 的斑点印迹法
  • 免疫组织化学(使用福尔马林固定、石蜡包埋切片,稀释度为 1:100)
  • 最低稀释度为 1:2,000 的直接 ELISA
还用于:
  • 免疫沉淀和免疫印迹
  • Ra2I 固相结合试验
  • Southwestern 印迹法
  • 蛋白质印迹法
  • 免疫细胞化学

生化/生理作用

抗生物素蛋白 (Avidin) 是一种四聚体或二聚体的生物素结合蛋白,产生于鸟类、爬行动物和两栖动物的输卵管中,并沉积在其蛋清中。由生物素的 4 个高亲和力结合位点组成。ExtrAvidin ® 由蛋清亲和素制备而成。它是亲和纯化亲和素的改良形式,具有亲和素的高比活性和链霉亲和素的低背景染色。是一种由 阿维迪链霉菌 产生的生物素结合蛋白。抗生物素蛋白-过氧化物酶可与生物素以不同的技术联合使用。

外形

含有0.05% MIT的0.01M磷酸盐缓冲盐水(pH7.4)溶液

制备说明

亲和纯化蛋白

储存及稳定性

储存在2-8 °C条件下。切勿冷冻。如果长期储存时出现轻微混浊,使用前通过离心澄清溶液。

法律信息

ExtrAvidin is a registered trademark of Sigma-Aldrich Co. LLC

免责声明

除非我们的产品目录或产品随附的其他公司文件中另有说明,否则我们的产品仅用于研究用途,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、体外或体内治疗用途或任何类型的人类或动物消费或应用。

象形图

Exclamation mark

警示用语:

Warning

危险声明

危险分类

Skin Sens. 1

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 2

闪点(F)

Not applicable

闪点(C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)


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