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生物源
human
品質等級
重組細胞
expressed in E. coli
化驗
>97% (SDS-PAGE)
形狀
lyophilized powder
效力
0.08-0.8 ng/mL EC50
分子量
~6 kDa
包裝
pkg of 200 and 500 μg
儲存條件
avoid repeated freeze/thaw cycles
雜質
≤1 EU/μg Endotoxin
顏色
white
溶解度
water: soluble 0.190-0.210, clear, colorless
UniProt登錄號
儲存溫度
−20°C
基因資訊
human ... EGF(1950)
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一般說明
表皮生长因子（EGF）在Henle′s环和肾远曲小管中合成。它也在唾液腺和十二指肠中产生。
表皮生长因子（EGF）是小的促有丝分裂多肽（∼6 6 kDa），存在于许多哺乳动物物种中，分布在许多组织和体液中。†人EGF与β-尿抑胃素相同，β-尿抑胃素是基于其胃酸分泌抑制能力而被识别和分离的多肽。† EGF是生长因子家族的成员，其特征在于存在6个保守的半胱氨酸基序，形成了三个二硫键。重组人EGF中3个链内二硫键的位置与小鼠EGF的位置相同。†EGF与牛痘病毒19 kDa蛋白中包含的序列同源，† 该病毒可能利用EGF受体进入细胞† EGF对多种表皮和上皮细胞具有促有丝分裂作用，包括成纤维细胞、神经胶质细胞、乳腺上皮细胞、血管和角膜内皮细胞、牛粒层细胞、兔软骨细胞、HeLa细胞和SV40-3T3细胞。†
應用
人表皮生长因子，不含动物成分，已用于：
- 用作在LHC-8培养基中的补充剂对肝细胞系进行培养
- 胎牛血清中（FBS）-杜氏改良必需培养基（DMEM）/F12培养基中，以用于人类神经胶质瘤细胞的原代培养
- 用作正常成纤维细胞（NFs）条件培养基中的添加剂，以研究其对子宫内膜癌细胞（EC）迁移和侵袭的影响
- 作为肿瘤球培养基的成分
生化／生理作用
表皮生长因子（EGF）有助于诱导细胞生长、增殖和分化。它可参与肾脏中肾组织的修复。它可借助瞬时受体电位阳离子通道6（TRMP6）促进镁的重吸收。EGF已被发现可参与药物性肾镁丢失的病理生理过程。
引用
1. Carpenter, G., and Cohen, S., Rapid enhancement of protein phosphorylation in A-431 cell membrane preparations by epidermal growth factor. Annu. Rev. Biochem., 48, 193-216 (1979).
2.Gregory, H., Isolation and structure of urogastrone and its relationship to epidermal growth factor. Nature, 257, 325-327 (1975).
3.George-Nascimento, C. et al., Characterization of recombinant human epidermal growth factor produced in yeast. Biochemistry, 27, 797-802 (1988).
4.Todaro, G.J. et al., Transforming growth factors produced by certain human tumor cells: polypeptides that interact with epidermal growth factor receptors. Proc. Natl. Acad. Sci. USA, 77, 5258-5262 (1980).
5.Blomquist, M.C. et al., Vaccinia virus 19-kilodalton protein: relationship to several mammalian proteins, including two growth factors. Proc. Natl. Acad. Sci. USA, 81, 7363-7367 (1984).
6.Eppstein, D.A. et al., Epidermal growth factor receptor occupancy inhibits vaccinia virus infection. Nature, 318, 663-665 (1985).
2.Gregory, H., Isolation and structure of urogastrone and its relationship to epidermal growth factor. Nature, 257, 325-327 (1975).
3.George-Nascimento, C. et al., Characterization of recombinant human epidermal growth factor produced in yeast. Biochemistry, 27, 797-802 (1988).
4.Todaro, G.J. et al., Transforming growth factors produced by certain human tumor cells: polypeptides that interact with epidermal growth factor receptors. Proc. Natl. Acad. Sci. USA, 77, 5258-5262 (1980).
5.Blomquist, M.C. et al., Vaccinia virus 19-kilodalton protein: relationship to several mammalian proteins, including two growth factors. Proc. Natl. Acad. Sci. USA, 81, 7363-7367 (1984).
6.Eppstein, D.A. et al., Epidermal growth factor receptor occupancy inhibits vaccinia virus infection. Nature, 318, 663-665 (1985).
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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