Merck

E6758

Sigma-Aldrich

乙二胺四乙酸

anhydrous, crystalline, BioReagent, suitable for cell culture

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别名:
EDTA, Ethylenedinitrilotetraacetic acid, 亚乙基二次氮基四乙酸, 依地酸
线性分子式:
(HO2CCH2)2NCH2CH2N(CH2CO2H)2
CAS号:
分子量:
292.24
Beilstein:
1716295
EC 号:
MDL编号:
eCl@ss:
39030907
PubChem化学物质编号:
NACRES:
NA.25

等级

anhydrous

产品线

BioReagent

检测方案

≥98.5%

形式

crystalline

reaction suitability

reagent type: chelator

technique(s)

cell culture | mammalian: suitable

pH值(酸碱度)

2.5 (23 °C, 10 g/L)

mp

250 °C (dec.) (lit.)

溶解性

3  M NaOH: 100 mg/mL

密度

1.46 g/cm3 at 20 °C

痕量阳离子

C: 40.7-41.5%
N: 9.3-9.8%

SMILES string

OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O

InChI

1S/C10H16N2O8/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20)

InChI key

KCXVZYZYPLLWCC-UHFFFAOYSA-N

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此商品
EDSE9884PHR2586
Ethylenediaminetetraacetic acid anhydrous, crystalline, BioReagent, suitable for cell culture

Sigma-Aldrich

E6758

乙二胺四乙酸

Essential+ Grade
Ethylenediaminetetraacetic acid BioUltra, anhydrous, ≥99% (titration)

Sigma-Aldrich

EDS

乙二胺四乙酸

Premium Grade
Ethylenediaminetetraacetic acid ACS reagent, 99.4-100.6%, powder

Sigma-Aldrich

E9884

乙二胺四乙酸

Essential Grade
Edetic Acid

Supelco

PHR2586

乙二胺四乙酸

-
product line

BioReagent

product line

BioUltra

product line

-

product line

-

assay

≥98.5%

assay

≥99% (titration)

assay

99.4-100.6%

assay

-

form

crystalline

form

powder

form

powder

form

solid

technique(s)

cell culture | mammalian: suitable

technique(s)

-

technique(s)

-

technique(s)

-

pH

2.5 (23 °C, 10 g/L)

pH

2.5 (23 °C, 10 g/L)

pH

2.5 (23 °C, 10 g/L)

pH

-

应用

乙二胺四乙酸已被用于:
  • 作为肽稀释缓冲液的组分,用于细胞内BH3(Bcl-2同源(BH)结构域)分析和验证
  • 作为肝星状细胞(HSC)培养的培养基成分
  • 作为眼组织蛋白质提取的匀浆缓冲液的组分
  • 用于阻止裂解缓冲液中的核酸酶反应

生化/生理作用

乙二胺四乙酸(EDTA)是一种抗凝血剂,主要用于预防血液样本凝结。EDTA可引发血小板聚集,导致假性血小板减少症。EDTA和四环素可一起用作根面处理剂并用于缓解牙龈感染。EDTA-四环素治疗可降低动脉粥样硬化中的冠状动脉钙水平。EDTA二钠钙给药可有效减缓慢性肾病的进展。

象形图

Exclamation mark

警示用语:

Warning

危险声明

危险分类

Eye Irrit. 2

储存分类代码

11 - Combustible Solids

WGK

WGK 2

闪点(F)

Not applicable

闪点(C)

Not applicable

个人防护装备

dust mask type N95 (US), Eyeshields, Gloves


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Intracellular BH3 Profiling Reveals Shifts in Antiapoptotic Dependency in Human B Cell Maturation and Mitogen-Stimulated Proliferation
Dai J and Luftig MA
Journal of Immunology, ji1701473-ji1701473 (2018)
Analysis of Direct Interaction between Viral DNA-binding Proteins by Protein Pull-down Co-immunoprecipitation Assay
Lechuga A, et al.
Bio-protocol, 8(1), 1571-1571 (2018)
Protein Expressions of the Small GTPase Rho Proteins in Pterygial Tissue and Leukocytes of Patients with Pterygium
Saracaloglu A, et al.
Proceedings, 2(25), 1571-1571 (2018)
The blockade of lipophagy pathway is necessary for docosahexaenoic acid to regulate lipid droplet turnover in hepatic stellate cells
Qiu S, et al.
Biomedicine and Pharmacotherapy, 109, 1841-1850 (2019)
Calcification in coronary artery disease can be reversed by EDTA-tetracycline long-term chemotherapy
Maniscalco BS and Taylor KA
Pathophysiology : The Official Journal of the International Society for Pathophysiology / ISP, 11(2), 95-101 (2004)

实验方案

Restriction Enzyme Cloning Manual Buffer Recipes

TE Buffer; Elution Buffer; 10x Ligation Buffer; 0.5 M PIPES Buffer; Inoue Transformation Buffer

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