Merck

E7885

Sigma-Aldrich

弹性蛋白酶 来源于猪胰腺

lyophilized powder, suitable for cell culture

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别名:
弹性蛋白酶 来源于猪胰腺, 胰酞酶 E
CAS号:
EC 号:
MDL编号:
NACRES:
NA.75

生物来源

Porcine

质量水平

形式

lyophilized powder

specific activity

≥4 units/mg protein

分子量

25.9 kDa

包装

pkg of 1 μmol

technique(s)

cell culture | mammalian: suitable

pH值(酸碱度)

8

适用性

suitable for

异质活性

Trypsin ≤50 BAEE units/mg protein

运输

dry ice

储存温度

−20°C

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此商品
E0258E1250E0127
form

lyophilized powder

form

lyophilized powder

form

suspension

form

lyophilized powder

specific activity

≥4 units/mg protein

specific activity

≥4.0 units/mg protein (biuret)

specific activity

≥4.0 units/mg protein

specific activity

≥4.0 units/mg protein

mol wt

25.9 kDa

mol wt

-

mol wt

25.9 kDa

mol wt

25.9 kDa

technique(s)

cell culture | mammalian: suitable

technique(s)

-

technique(s)

-

technique(s)

-

pH

8

pH

-

pH

-

pH

-

一般描述

弹性蛋白酶是 240 个氨基酸残基的单一多肽链,含有四个二硫键。分子量约为 25.9kDa。该酶被合成为无活性的酶原,原弹性蛋白酶,其通过胰蛋白酶在 N-末端的有限蛋白水解而转化为活性形式。

应用

来自猪胰腺的弹性蛋白酶用于以下应用:
  • 细胞培养技术
  • ELISA 技术
  • 用于测定弹性蛋白酶抑制活性

生化/生理作用

弹性蛋白酶水解弹性蛋白(弹性纤维的特定蛋白质)并消化血红蛋白、酪蛋白和纤维蛋白。
降低弹性蛋白的酶,弹性蛋白是参与拉伸过程的组织的基质成分。它是一种具有广泛特异性的丝氨酸蛋白酶。它切割小疏水性氨基酸(如 Ile、Gly、Ala、Ser、Val 和 Leu)的羧基侧的蛋白质。该酶还水解酰胺和酯,例如 N-苯甲酰基-L-丙氨酸甲酯。

包装

包装尺寸基于蛋白质含量

单位定义

一个单元将在 25℃ 下,每分钟,pH8.0,水解 1.0 μmole 的 N-琥珀酰-L-Ala-Ala-Ala-对硝基苯胺。

象形图

Exclamation markHealth hazard

警示用语:

Danger

危险分类

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

靶器官

Respiratory system

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(F)

Not applicable

闪点(C)

Not applicable


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Scott W Walsh et al.
Reproductive sciences (Thousand Oaks, Calif.), 27(1), 246-259 (2020-02-13)
Decidual macrophages secrete proteases that activate protease-activated receptor 1 (PAR-1). We hypothesized that activation of the inflammatory response by bacteria is amplified by proteases, initiating labor. In addition, we hypothesized that commensal bacteria trigger an inflammatory response by activating NF-κB
Adrien Lupieri et al.
Cardiovascular research, 116(2), 438-449 (2019-05-21)
Defects in efficient endothelial healing have been associated with complication of atherosclerosis such as post-angioplasty neoatherosclerosis and plaque erosion leading to thrombus formation. However, current preventive strategies do not consider re-endothelialization in their design. Here, we investigate mechanisms linking immune
George Butler et al.
Proceedings. Biological sciences, 287(1940), 20202523-20202523 (2020-12-02)
An important question in cancer evolution concerns which traits make a cell likely to successfully metastasize. Cell motility phenotypes, mediated by cell shape change, are strong candidates. We experimentally evolved breast cancer cells in vitro for metastatic capability, using selective
F Seibold et al.
Gut, 32(10), 1192-1197 (1991-10-01)
The presence of antibodies against pancreatic juice (PAB) in patients with Crohn's disease has recently been reported. In our study sera from 273 patients with inflammatory bowel disease (222 with Crohn's disease, 51 with ulcerative colitis) have been examined for
Yoshiyuki Okumura et al.
Nihon Ishinkin Gakkai zasshi = Japanese journal of medical mycology, 48(1), 13-18 (2007-02-09)
The amino acid sequence of elastase inhibitor, AFLEI, isolated from Aspergillus flavus was determined by the Edman sequencing procedure of peptides derived from digests utilizing clostripain. A molecular weight of 7,525.8 was observed by TOF-MS. AFLEI contained 68 amino acid

实验方案

Enzymatic Assay of Elastase (EC 3.4.21.36)

Enzymatic Assay of Elastase (EC 3.4.21.36), a continuous spectrophotometric rate determination.

Chromatograms

application for HPLC

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