Merck

F0397

Sigma-Aldrich

Anti-Cytokeratin pan-FITC antibody, Mouse monoclonal

clone PCK-26, purified from hybridoma cell culture

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别名:
Anti-CK16-Fluorescein isothiocyanate, Anti-FNEPPK, Anti-K16, Anti-K1CP, Anti-KRT16A, Anti-NEPPK, Anti-PC1
NACRES:
NA.41

生物来源

mouse

偶联物

FITC conjugate

抗体形式

purified from hybridoma cell culture

antibody product type

primary antibodies

克隆

PCK-26, monoclonal

形式

buffered aqueous solution

technique(s)

direct immunofluorescence: 1:25 using formalin-fixed, paraffin-embedded sections of human or animal tissues
immunohistochemistry (frozen sections): suitable

运输

dry ice

储存温度

−20°C

target post-translational modification

unmodified

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此商品
F3418P2871F4772
conjugate

FITC conjugate

conjugate

FITC conjugate

conjugate

unconjugated

conjugate

FITC conjugate

antibody form

purified from hybridoma cell culture

antibody form

purified immunoglobulin

antibody form

purified from hybridoma cell culture

antibody form

purified immunoglobulin

clone

PCK-26, monoclonal

clone

C-11, monoclonal

clone

C-11, monoclonal

clone

CY-90, monoclonal

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

technique(s)

direct immunofluorescence: 1:25 using formalin-fixed, paraffin-embedded sections of human or animal tissues, immunohistochemistry (frozen sections): suitable

technique(s)

direct immunofluorescence: 1:50 using PtK2 cells, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50 using human placenta, western blot: suitable

technique(s)

immunocytochemistry: 15-30 μg/mL using methanol/acetone-fixed PTK-2 cells or A549 - human lung carcinoma cells, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 15-30 μg/mL using human skin, immunohistochemistry (frozen sections): suitable, microarray: suitable, western blot: suitable

technique(s)

direct immunofluorescence: 1:100 using formalin-fixed, paraffin-embedded sections of human or animal tissues, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable

一般描述

Cytokeratins are proteins of keratin-containing intermediate filaments that provide mechanical support and other additional functions in epithelial cells. It is found in the intracytoplasmic cytoskeleton of epithelial tissue. Epithelial tissue expresses cytokeratin subunits in a specific and stable pattern. Cytokeratins along with vimentin are involved in cell proliferation, migration and differentiation of preodontoblasts and preameloblasts. The intermediate-sized filaments are abundant in human endothelial cells and are mostly of vimentin type.
FITC Monoclonal Anti-Pan Cytokeratin is a purified mouse monoclonal antibody conjugated with fluorescein isothiocyanate (FITC) isomer I. Monoclonal anti-pan cytokeratin (mouse IgG1 isotype) is derived from the PCK-26 hybridoma produced by the fusion of mouse myeloma cells and splenocytes of immunized BALB/c mice. Cytokeratins, a group comprised of at least 29 different proteins are characteristic of epithelial and trichocytic cells. Cytokeratin 5, 6 and 8 are members of the type II, neutral-to-basic subfamily. Cytokeratin peptide 5 is the primary type II keratin in stratified epithelia while cytokeratin type 8 is a major type II keratin in simple epithelia. Cytokeratin 6 is a "hyperproliferation" cytokeratin expressed in tissues with natural or pathological high turnover.

特异性

The antibody recognizes an epitope located on the Type II cytokeratins 1, 5, 6, and 8. PCK-26 is a broad spectrum antibody which reacts specifically with a variety of normal, reactive, and neoplastic epithelial tissues. The antibody reacts with simple, cornifying, and non-cornifying squamous epithelia and pseudostratified epithelia.

免疫原

cytokeratin from human epidermis.

应用

Monoclonal Anti-Cytokeratin, pan-FITC antibody produced in mouse has been used in:
  • immunohistochemistry
  • staining chicken intestinal cells
  • immunofluorescence staining

Monoclonal Anti-Cytokeratin, pan-FITC antibody produced in mouse is suitable for direct immunofluorescence at a dilution of 1:25 using formalin-fixed, paraffin-embedded sections of human or animal tissues and immunohistochemistry (frozen sections).

外形

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存分类代码

10 - Combustible liquids

WGK

nwg

闪点(F)

Not applicable

闪点(C)

Not applicable

个人防护装备

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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