Merck
  • F1269
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F1269

Sigma-Aldrich

Anti-Avidin–FITC antibody, Mouse monoclonal

clone WC19.10, purified from hybridoma cell culture

All Photos(1)
Synonym(s):
Monoclonal Anti-Avidin

生物来源

mouse

质量水平

200

偶联物

FITC conjugate

抗体形式

purified immunoglobulin

antibody product type

primary antibodies

克隆

WC19.10, monoclonal

形式

buffered aqueous solution

储存条件

protect from light

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
indirect immunofluorescence: suitable

同位素/亚型

IgG1

运输

dry ice

储存温度

−20°C

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抗 CD4-小鼠单克隆 FITC 抗体

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

conjugate

FITC conjugate

conjugate

FITC conjugate

conjugate

-

conjugate

FITC conjugate

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified from hybridoma cell culture

antibody form

purified immunoglobulin

clone

WC19.10, monoclonal

clone

AC-15, monoclonal

clone

FL-D6, monoclonal

clone

Q4120, monoclonal

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, indirect immunofluorescence: suitable

technique(s)

direct immunofluorescence: 1:250 using cultured human and chicken fibroblasts

technique(s)

immunoblotting: 1.25-2.5 μg/mL using ExtrAvidin-FITC, immunohistochemistry: suitable, indirect ELISA: 0.15-0.3 μg/mL using 5 μg/ml ExtrAvidin-FITC

technique(s)

flow cytometry: 10 μL using 1 × 106 cells

一般描述

Monoclonal Anti-Avidin (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Avidin is a glycoprotein (68 kDa) composed of four identical polypeptide chains isolated from egg whites.
The antibody binds to the avidin molecule without interfering with the biotin-binding reaction. The product reacts specifically with egg white avidin, ExtrAvidin and their complexes formed with biotin. The antibody shows no cross-reaction with streptavidin.

特异性

FITC conjugated monoclonal anti-avidin may be used for single or double immunofluorescence labeling techniques and for modification of non-fluorescent to fluorescent reactions.

免疫原

Purified egg white avidin.

应用

Anti-Avidin-FITC antibody, Mouse monoclonal has been used in indirect immunohistology.

生化/生理作用

Avidin has a high binding affinity for the vitamin biotin. This strong interaction has been exploited in the design of immunoassays and immunohistologic staining techniques to serve as a basis for identifying antigen antibody interactions

外形

Solution supplied in 0.01 M phosphate buffered saline, pH 8.0, containing 1% BSA and 15 mM sodium azide.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存分类代码

10 - Combustible liquids

WGK

nwg

闪点(F)

Not applicable

闪点(C)

Not applicable

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