Merck
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F1804

Sigma-Aldrich

单克隆抗-FLAG® M2 小鼠抗

1 mg/mL, clone M2, affinity isolated antibody, buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4)

Synonym(s):
flag抗体, 抗 ddddk, 抗 dykddddk
NACRES:
NA.32

生物来源

mouse

质量水平

偶联物

unconjugated

抗体形式

affinity isolated antibody

antibody product type

primary antibodies

克隆

M2, monoclonal

形式

buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4)

纯化方式

affinity chromatography (affinity tag purification)

species reactivity

all

浓度

1 mg/mL

同位素/亚型

IgG1

免疫原序列

DYKDDDDK

运输

wet ice

储存温度

−20°C

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F3040F3165F4042
biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin (Purified IgG1 subclass)

antibody form

purified immunoglobulin

clone

M2, monoclonal

clone

M1, monoclonal

clone

M2, monoclonal

clone

M5, monoclonal

form

buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4)

form

buffered aqueous solution

form

buffered aqueous solution (10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide)

form

buffered aqueous solution

species reactivity

all

species reactivity

all

species reactivity

all

species reactivity

all

一般描述

小鼠抗-FLAG M2抗体,可与含有FLAG肽序列结合的亲和纯化单克隆抗体。该抗体可识别FLAG肽序列的N-末端、Met-N-末端、C-末端和融合蛋白的内部位点。

纯化方法 - 亲和标签纯化

特异性

结合位点:N-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-C

免疫原

FLAG;肽序列DYKDDDDK

应用

用于通过免疫印迹、免疫沉淀(IP)、免疫组化、免疫荧光及免疫细胞化学进行FLAG融合蛋白的高灵敏度及特异性检测。 优化用于在哺乳动物、植物和细菌表达系统中进行FLAG融合蛋白的单条带检测。

Western Blotting和EIA

通过我们的FLAG®文献端口浏览更多应用参考文献。

外形

在磷酸盐缓冲液中含有50%甘油。

制备说明

在立即使用前用3%脱脂牛奶稀释于Tris缓冲盐溶液(TBS),pH 8.0

法律信息

ANTI-FLAG is a registered trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Sigma-Aldrich Co. LLC

储存分类代码

10 - Combustible liquids

WGK

WGK 2

闪点(F)

Not applicable

闪点(C)

Not applicable


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