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F3165

Sigma-Aldrich

单克隆抗-FLAG® M2 小鼠抗

clone M2, purified immunoglobulin (Purified IgG1 subclass), buffered aqueous solution (10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide)

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别名:
抗 ddddk, 抗 dykddddk
NACRES:
NA.32

生物来源

mouse

质量水平

200

偶联物

unconjugated

抗体形式

purified immunoglobulin (Purified IgG1 subclass)

antibody product type

primary antibodies

克隆

M2, monoclonal

形式

buffered aqueous solution (10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide)

纯化方式

using Protein A

species reactivity

all

浓度

3.8-4.2 mg/mL

technique(s)

western blot: 10 μg/mL (Protein A)

同位素/亚型

IgG1

免疫原序列

DYKDDDDK

运输

dry ice

储存温度

−20°C

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F1804F4049F3040
Sigma-Aldrich

Sigma-Aldrich

F3165

单克隆抗-FLAG® M2 小鼠抗

Monoclonal ANTI-FLAG® M2 antibody produced in mouse 1 mg/mL, clone M2, affinity isolated antibody, buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4)

Sigma-Aldrich

F1804

单克隆抗-FLAG® M2 小鼠抗

Monoclonal ANTI-FLAG® M2-FITC antibody produced in mouse clone M2, purified immunoglobulin, buffered aqueous solution

Sigma-Aldrich

F4049

FLAG® M2-FITC单克隆抗体 小鼠抗

Sigma-Aldrich

Sigma-Aldrich

F3040

Anti-FLAG® M1小鼠单抗 小鼠抗

conjugate

unconjugated

conjugate

unconjugated

conjugate

FITC conjugate

conjugate

unconjugated

antibody form

purified immunoglobulin (Purified IgG1 subclass)

antibody form

affinity isolated antibody

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

clone

M2, monoclonal

clone

M2, monoclonal

clone

M2, monoclonal

clone

M1, monoclonal

form

buffered aqueous solution (10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide)

form

buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4)

form

buffered aqueous solution

form

buffered aqueous solution

species reactivity

all

species reactivity

all

species reactivity

all

species reactivity

all

一般描述

抗-Flag M2抗体用于检测Flag融合蛋白质。该单克隆产生于小鼠中并可识别FLAG序列的N-末端、Met N-末端和C-末端。该抗体还可在内部位点识别FLAG。不同于M1，M2不是钙依赖性的。
F3165使用蛋白质A树脂进行亲和纯化，因此不仅含有抗-FLAG M2抗体，还有少量的原生小鼠IgG，这会增加它在大多数应用中的敏感性。
纯化方法——蛋白质A

免疫原

FLAG；肽序列DYKDDDDK

应用

小鼠产生的 ANTI-FLAG® M2 单克隆抗体已用于：
  • 免疫印迹
  • 免疫沉淀
  • 免疫细胞化学
  • 免疫荧光
  • ELISA
  • EIA
  • 染色质免疫沉淀
  • 电子显微分析
  • 流式细胞分析
  • 高速迁移测定

浏览我们有关其他应用的参考文献，请访问 FLAG® 文献页面。

制备说明

用3%脱脂牛奶在Tris缓冲盐溶液（TBS），pH 8.0中将抗体从0.5-10 ug/mL进行稀释

储存及稳定性

将稀释后的抗体分装并-20°C储存。不建议反复冻融。
注意：随着时间流逝，由于分子间的疏水相互作用，少量纯化抗体会从溶液中沉淀出来。如果在该产品中观察到沉淀，请短暂离心小瓶以复溶沉淀。从澄清上清液中取出所需体积的抗体溶液备用。这不会改变纯化抗体在蛋白质印迹或免疫沉淀应用中的性能。

法律信息

ANTI-FLAG is a registered trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Sigma-Aldrich Co. LLC

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产品编号
说明
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储存分类代码

12 - Non Combustible Liquids

WGK

nwg

闪点(F)

Not applicable

闪点(C)

Not applicable

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Hong Zhu et al.
Molecular biology of the cell, 24(11), 1619-1637 (2013-04-12)
Charcot-Marie-Tooth (CMT) disease is an inherited neurological disorder. Mutations in the small integral membrane protein of the lysosome/late endosome (SIMPLE) account for the rare autosomal-dominant demyelination in CMT1C patients. Understanding the molecular basis of CMT1C pathogenesis is impeded, in part
T P Molitor et al.
Oncogenesis, 2, e48-e48 (2013-06-05)
The vaccinia-related kinases (VRKs) comprise a branch of the casein kinase family. VRK1, a ser/thr kinase with a nuclear localization, is the most well-studied paralog and has been described as a proproliferative protein. In lower eukaryotes, a loss of VRK1
Fangzhi Tan et al.
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