Merck

F5636

Sigma-Aldrich

单克隆抗-FITC 小鼠抗

clone FL-D6, ascites fluid

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别名:
单克隆抗-FITC
NACRES:
NA.46

生物来源

mouse

偶联物

unconjugated

抗体形式

ascites fluid

antibody product type

primary antibodies

克隆

FL-D6, monoclonal

包含

15 mM sodium azide

technique(s)

indirect ELISA: 1:2,500 using FITC-conjugate
indirect immunofluorescence: suitable

同位素/亚型

IgG1

运输

dry ice

储存温度

−20°C

target post-translational modification

unmodified

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此商品
A1812SAB4200738F4049
Monoclonal Anti-FITC antibody produced in mouse clone FL-D6, ascites fluid

Sigma-Aldrich

F5636

单克隆抗-FITC 小鼠抗

Monoclonal Anti-FITC−Alkaline Phosphatase antibody produced in mouse clone FL-D6, purified immunoglobulin, buffered aqueous solution

Sigma-Aldrich

A1812

单克隆抗-FITC

conjugate

unconjugated

conjugate

alkaline phosphatase conjugate

conjugate

-

conjugate

FITC conjugate

antibody form

ascites fluid

antibody form

purified immunoglobulin

antibody form

purified from hybridoma cell culture

antibody form

purified immunoglobulin

clone

FL-D6, monoclonal

clone

FL-D6, monoclonal

clone

FL-D6, monoclonal

clone

M2, monoclonal

technique(s)

indirect ELISA: 1:2,500 using FITC-conjugate, indirect immunofluorescence: suitable

technique(s)

dot blot: 1:80,000 using human IgG/FITC monoclonal anti-human IgG, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:20 using FITC conjugate, indirect ELISA: 1:30,000

technique(s)

immunoblotting: 1.25-2.5 μg/mL using ExtrAvidin-FITC, immunohistochemistry: suitable, indirect ELISA: 0.15-0.3 μg/mL using 5 μg/ml ExtrAvidin-FITC

technique(s)

direct immunofluorescence: 10 μg/mL using mammalian cells fixed with methanol:acetone

isotype

IgG1

isotype

IgG1

isotype

IgG1

isotype

IgG1

一般描述

FITC(异硫氰酸荧光素)是一种荧光染料,可吸收紫外线或蓝光,使分子激发并发出可见的黄绿色光。 该发射在去除引起激发的光后停止。 当反应物之一是细胞、组织或其他生物结构的一部分时,荧光染料标记可快速、准确地定位抗原-抗体相互作用。 FITC是免疫荧光技术中常用的抗体标记物,因为FITC与蛋白质的结合相对容易,并且通常不会破坏标记蛋白的生物活性。 FITC作为半抗原被广泛用于标记不同的蛋白质。 FITC抗体用于鉴定FITC标记的蛋白质,并作为模型来研究抗体对明确定义的半抗原的反应机制。

特异性

小鼠单克隆克隆FL-D6抗FITC抗体将与游离或偶联FITC反应。该抗体未与结合的或游离的TRITC(异硫氰酸四甲基罗丹明)反应。
该抗体可与游离的和结合的异硫氰酸荧光素(FITC)反应。

免疫原

FITC-BSA偶联物

应用

小鼠单克隆克隆FL-D6抗FITC抗体可用于检测FITC,并作为通用指示剂,用于将FITC与其他免疫化学试剂桥接。 可用于ELISA、竞争ELISA和免疫荧光技术。 FITC抗FITC系统已用于放大免疫荧光检测中的信号,并通过亲和色谱法将结合物与游离示踪剂分离。 该抗体也可用于分离在其表面上具有FITC标记配体的细胞。

免责声明

除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

储存分类代码

10 - Combustible liquids

WGK

nwg

闪点(F)

Not applicable

闪点(C)

Not applicable


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