F6796

Sigma-Aldrich

Anti-Fibroblast Growth Factor Receptor-2, Extracellular antibody produced in rabbit

enhanced validation

affinity isolated antibody, buffered aqueous solution

别名:
Anti-FGFR-2
MDL number:
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 115-125 kDa (doublet)

species reactivity

human

packaging

antibody small pack of 25 μL

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

application(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,000 using trypsin-digested, human and animal tissue sections
western blot: 1:2,000 using extract of FGFR-2 transiently transfected cells

conjugate

unconjugated

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... FGFR2(2263)

General description

Fibroblast growth factors (FGFs) belongs to the large tyrosine kinase family of growth factor receptors (17-38kDa). The FGF family consists of at least seventeen members designated FGF-1 through FGF-17 that bind to high affinity FGF receptors (FGFRs). Soluble, secreted or possibly cleaved forms of FGFR-1 and FGFR-2 have also been found in body fluids. They are glycosylated 110-150 kDa proteins that are constructed of an extracellular ligand binding region with either two, or typically three, immunoglobulin (Ig)-like domains and an eight amino acid "acidic box", a transmembrane region and a cytoplasmic split tyrosine kinase domain that is activated following ligand binding and receptor dimerization. The ligand binding site of all FGFRs is confined to the extracellular Ig-like domains 2 and 3.

Specificity

The antibody specifically reacts with human FGFR-2; no reaction with FGFR-1 or FGFR-3 is detected.
The antibody detects human FGFR-2 (bek) in extracts of transfected cells.

Immunogen

synthetic peptide corresponding to amino acids 362-374 of the extracellular region of human FGFR-2 (Bek).

Application

Anti-Fibroblast Growth Factor Receptor-2, Extracellular antibody produced in rabbit has been used in immunoprecipitation and immunoblotting.

Packaging

0.2 mL in glass insert

Biochem/physiol Actions

Fibroblast growth factors (FGFs) are potent physiological regulators normal development, wound healing and repair, angiogenesis, a variety of neurotrophic activities, in hematopoiesis as well as in tissue remodeling and maintenance. FGFRs exhibit overlapping recognition and redundant specificity. Signal transduction by FGFRs requires dimerization or oligomerization and autophosphorylation of the receptors through their tyrosine kinase domain. Subsequent association with cytoplasmic signaling molecules leads to DNA synthesis or differentiation. The signaling and biological responses elicited by distinct FGFRs substantially differ. FGFR-2 is highly expressed in developing human tissues including the brain (preferentially in glial cells), choroid plexus, skin, lung, kidney and bone. It may be found in several anchorage-dependent cells, such as normal and malignant breast cancer cells.

Physical form

Solution in 0.01 M phosphate buffered saline,
pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide

Preparation Note

Affinity isolated on a peptide-agarose column.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Y Zimmer et al.
The Journal of biological chemistry, 268(11), 7899-7903 (1993-04-15)
The murine fibroblast growth factor receptor 2 (FGFR2) and keratinocyte growth factor receptor (KGFR) are two products of the same gene which display distinct binding specificities. We and others have shown that a major structural element underlying this functional divergence...
Takahiro Sawada et al.
Genes to cells : devoted to molecular & cellular mechanisms, 15(3), 297-311 (2010-02-27)
EphA4 belongs to a superfamily of receptor tyrosine kinases and interacts with several molecules including fibroblast growth factor receptors (FGFRs) as we reported earlier. Several receptor tyrosine kinases, FGFRs, Trks, Alk and Ret, are currently known to transduce a signal...
Fibroblast growth factor 23 reduces expression of type IIa Na+/Pi co-transporter by signaling through a receptor functionally distinct from the known FGFRs in opossum kidney cells
Yan X, et al.
Genes Cells, 10(5), 489-502 (2005)
Ternary complex formation of EphA4, FGFR and FRS2$\alpha$ plays an important role in the proliferation of embryonic neural stem/progenitor cells
Sawada T, et al.
Genes Cells, 15(3), 297-311 (2010)
D Givol et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 6(15), 3362-3369 (1992-12-01)
Since 1989, the receptors for fibroblast growth factors (FGFs) were cloned and characterized as a subgroup of the family of receptor tyrosine kinases. Four FGF receptor genes were identified, all of which encode membrane-bound glycoproteins containing three immunoglobulin (Ig) -like...

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