F7250
荧光素异硫氰酸酯异构体I
suitable for protein labeling, ≥90% (HPLC), powder
Recommended Products
检测方案
≥90% (HPLC)
形式
powder
mp
>360 °C (lit.)
溶解性
acetone: 1 mg/mL
荧光
λex 492 nm; λem 518 nm (green)
适用性
suitable for protein labeling
application(s)
diagnostic assay manufacturing
hematology
histology
储存温度
2-8°C
SMILES string
Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5cc(ccc45)N=C=S)c1
InChI
1S/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H
InChI key
MHMNJMPURVTYEJ-UHFFFAOYSA-N
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This Item | F6377 | F2456 | F4274 |
|---|---|---|---|
| assay ≥90% (HPLC) | assay - | assay - | assay ≥97.5% (HPLC) |
| form powder | form powder | form powder | form powder |
| mp >360 °C (lit.) | mp >300 °C | mp 320 °C (lit.) | mp >360 °C (lit.) |
| solubility acetone: 1 mg/mL | solubility - | solubility - | solubility acetone: soluble 10 mg/mL |
| fluorescence λex 492 nm; λem 518 nm (green) | fluorescence λex 460 nm; λem 515 nm(lit.) | fluorescence - | fluorescence λex 492 nm; λem 518 nm (green) |
| suitability suitable for protein labeling | suitability - | suitability - | suitability - |
一般描述
它被广泛用于通过胺基将荧光标记附着到蛋白质上。异硫氰酸酯基团可与蛋白中的氨基末端和伯胺反应。它已用于对蛋白质标记，包括抗体和凝集素。
异硫氰酸荧光素异构体I已被提议作为接触敏化剂。
应用
生物学应用，包括用于蛋白荧光标记试剂、用于蛋白示踪的荧光试剂以及作为病原体快速鉴定的荧光抗体技术中的一种试剂。在毛细管电泳（CE）测定过程中，它可用作人尿液中苯丙胺、甲基苯丙胺、3,4-亚甲二氧基甲基苯丙胺和对苯乙胺的衍生试剂。它可用于荧光抗体的制备。它可用于体外敏化研究。
警示用语：
Danger
危险声明
危险分类
Resp. Sens. 1 - Skin Sens. 1
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(F)
Not applicable
闪点(C)
Not applicable
个人防护装备
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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