Merck
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F7250

Sigma-Aldrich

荧光素异硫氰酸酯异构体I

suitable for protein labeling, ≥90% (HPLC), powder

Synonym(s):
FITC, 荧光素5-异硫氰酸酯
Empirical Formula (Hill Notation):
C21H11NO5S
CAS Number:
Molecular Weight:
389.38
Beilstein:
1407295
EC 号:
MDL编号:
PubChem化学物质编号:
NACRES:
NA.47

质量水平

200

测定

≥90% (HPLC)

形式

powder

mp

>360 °C (lit.)

溶解性

acetone: 1 mg/mL

荧光

λex 492 nm; λem 518 nm (green)

适用性

suitable for protein labeling

application(s)

diagnostic assay manufacturing
hematology
histology

储存温度

2-8°C

SMILES string

Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5cc(ccc45)N=C=S)c1

InChI

1S/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H

InChI key

MHMNJMPURVTYEJ-UHFFFAOYSA-N

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一般描述

荧光素异硫氰酸酯(FITC)具有黄橘色的颜色以及在495 nm处的最大吸光值。通过激发,它可以散发出在525 nm具有最大发射的黄绿色。
它被广泛用于通过胺基将荧光标记附着到蛋白质上。异硫氰酸酯基团可与蛋白中的氨基末端和伯胺反应。它已用于对蛋白质标记,包括抗体和凝集素。
异硫氰酸荧光素异构体I已被提议作为接触敏化剂。

应用

用于蛋白FITC标记的试剂;蛋白和肽的微测序(HPLC)
生物学应用,包括用于蛋白荧光标记试剂、用于蛋白示踪的荧光试剂以及作为病原体快速鉴定的荧光抗体技术中的一种试剂。在毛细管电泳(CE)测定过程中,它可用作人尿液中苯丙胺、甲基苯丙胺、3,4-亚甲二氧基甲基苯丙胺和对苯乙胺的衍生试剂。它可用于荧光抗体的制备。它可用于体外敏化研究。
用于蛋白FITC标记的试剂;蛋白和肽的微测序(HPLC)

包装

50, 100, 250, 500 mg in glass bottle
1, 5 g in glass bottle

象形图

Health hazard

警示用语:

Danger

危险声明

危险分类

Resp. Sens. 1 - Skin Sens. 1

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(F)

Not applicable

闪点(C)

Not applicable

个人防护装备

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Certificate of Analysis

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Certificate of Origin

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