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Merck

F7425

抗-FLAG® 兔抗

affinity isolated antibody, buffered aqueous solution

别名:

抗-ddddk, 抗-dykddddk

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关于此项目

UNSPSC Code:
12352203
NACRES:
NA.32
MDL number:
MFCD02262912

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产品名称

抗-FLAG® 兔抗, affinity isolated antibody, buffered aqueous solution

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

all

concentration

~0.8 mg/mL

technique(s)

dot blot: 1-2.5 μg/mL
immunoprecipitation (IP): 4-8 μg using amino terminal FLAG-BAP fusion protein from E. coli crude lysate
indirect immunofluorescence: 5-10 μg/mL using 293T cells transfected with a plasmid encoding FLAG-JNK
western blot (chemiluminescent): 1-2.5 μg/mL using an E. coli periplasmic extract expressing an N-terminal FLAG fusion protein

isotype

IgG

immunogen sequence

DYKDDDDK

shipped in

dry ice

storage temp.

−20°C

Quality Level

200

相关类别

Application

采用斑点印迹、免疫印迹、免疫沉淀和免疫细胞化学检测，该抗体可以识别N-末端或C-末端FLAG标记融合蛋白上的FLAG 表位。
请访问我们的 FLAG® 应用门户网站，了解更多产品详情。

Biochem/physiol Actions

FLAG表位在融合蛋白的免疫亲和纯化中具有重要作用。

General description

FLAG表位是一种带有肠激酶切割位点的八氨基酸蛋白。
兔抗-FLAG多克隆亲核抗体抗-FLAG可识别位于FLAG融合蛋白上的FLAG表位。该抗体可与N-末端、N末端-Met及C末端FLAG融合蛋白反应。

Immunogen

FLAG；肽序列DYKDDDDK

Other Notes

2024年CiteAb奖——成功的帕金森研究供应商(2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research)

Physical form

含有1％牛血清白蛋白和15mM 叠氮化钠的0.01M磷酸盐缓冲盐水（pH7.4）中的溶液

Preparation Note

在可承受免疫肽的色谱柱上通过亲和色谱进行纯化。

Legal Information

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

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存储类别

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000485141
0000485141
0000422515
0000422515
0000395474

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Datasheet - F7425

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