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Merck

F7425

Millipore

抗-FLAG® 兔抗

affinity isolated antibody, buffered aqueous solution

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别名:
抗-ddddk, 抗-dykddddk
MDL號碼:
分類程式碼代碼:
12352203
NACRES:
NA.32

生物源

rabbit

品質等級

共軛

unconjugated

抗體表格

affinity isolated antibody

抗體產品種類

primary antibodies

無性繁殖

polyclonal

形狀

buffered aqueous solution

物種活性

all

濃度

~0.8 mg/mL

技術

dot blot: 1-2.5 μg/mL
immunoprecipitation (IP): 4-8 μg using amino terminal FLAG-BAP fusion protein from E. coli crude lysate
indirect immunofluorescence: 5-10 μg/mL using 293T cells transfected with a plasmid encoding FLAG-JNK
western blot (chemiluminescent): 1-2.5 μg/mL using an E. coli periplasmic extract expressing an N-terminal FLAG fusion protein

同型

IgG

免疫原序列

DYKDDDDK

運輸包裝

dry ice

儲存溫度

−20°C

一般說明

FLAG表位是一种带有肠激酶切割位点的八氨基酸蛋白。
兔抗-FLAG多克隆亲核抗体抗-FLAG可识别位于FLAG融合蛋白上的FLAG表位。该抗体可与N-末端、N末端-Met及C末端FLAG融合蛋白反应。

免疫原

FLAG;肽序列DYKDDDDK

應用

采用斑点印迹、免疫印迹、免疫沉淀和免疫细胞化学检测,该抗体可以识别N-末端或C-末端FLAG标记融合蛋白上的FLAG 表位。
请访问我们的 FLAG® 应用门户网站,了解更多产品详情。

生化/生理作用

FLAG表位在融合蛋白的免疫亲和纯化中具有重要作用。

外觀

含有1%牛血清白蛋白和15mM 叠氮化钠的0.01M磷酸盐缓冲盐水(pH7.4)中的溶液

準備報告

在可承受免疫肽的色谱柱上通过亲和色谱进行纯化。

法律資訊

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

水污染物質分類(WGK)

nwg

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析证书(COA)

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