Merck

FITC1

Sigma-Aldrich

FluoroTagFITC偶联试剂盒

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别名:
FITC
NACRES:
NA.32

包装

pkg of (Kit contains reagents for 5 conjugations.)

荧光

λex 495 nm; λem 525 nm

储存温度

2-8°C

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此商品
SBR00028SAB3700662SAB3701081
FITC labeled vancomycin for fluorescent microbial imaging

Sigma-Aldrich

SBR00028

FITC labeled vancomycin

fluorescence

λex 495 nm; λem 525 nm

fluorescence

-

fluorescence

-

fluorescence

-

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

200

Quality Level

100

Quality Level

100

Quality Level

100

应用

Flourotag FITC 偶联试剂盒可以用于流式细胞仪免疫组织化学和免疫细胞化学。它还可以用于FITC和多肽激素,细胞活素,生长因子和蛋白质偶联。

特点和优势

  • 适于少量(1 mg)和大量(5 mg)偶联物。
  • 完整水相法 - 不需要DMF
  • 从过量的FITC快速凝胶过滤分离偶联物
  • 偶联物和F/P比测定完整实验方案
  • 试剂足够至少进行5次5mg蛋白质的偶联反应,可供在扩大化前确定最佳F/P比。
  • 应用和实验方案参考

原理

Sigma提供方便的试剂盒用于制备FITC-标记抗体。异硫氰基荧光素(FITC),异构体1,是一种广泛使用的荧光团,因为在共轭反应时它有高量子效率和稳定性。FITC具有黄橘色的颜色以及在495 nm处的最大吸光值。通过激发,它可以散发出在525 nm具有最大发射的黄绿色。通过蛋白质或多肽链的自由氨基基团发生共轭反应,形成稳定的硫脲键(看反应)。FITC和抗体,凝集素,激素和生长因子共轭,用于各种免疫组织化学和流式细胞仪实验中。该实验方案对于抗体是最优方案,但用于其他蛋白质时需要用户自行修改。

分析说明

过程
1.在碳酸盐-重碳酸盐缓冲液中溶解蛋白质和FITC。
2.边搅拌边缓慢将FITC加入蛋白质。盖上箔片,在室温下搅拌2小时。
3.在G-25柱上将自由FITC和偶联物分开。收集组分。
4.收集含有偶联物的组分。
5.分光光度法确定F/P偶联物比例。
6.用1%牛血清白蛋白和0.1%叠氮钠稳定,0-5℃储存。

法律信息

FluoroTag is a trademark of Sigma-Aldrich Co. LLC

仅试剂盒组分

产品编号
说明

  • Sephadex G-25 column, 3.5 mL 1

  • Sephadex G-25 column, 9.1 mL 1

  • Fluorescein isothiocyanate isomer I - F7250KC-2MG 2 mg

试剂盒组分也可单独购买

产品编号
说明
化学品安全说明书

  • P3813Phosphate buffered saline, powder, pH 7.4, for preparing 1 L solutions 5 pkg化学品安全说明书

象形图

Exclamation mark

警示用语:

Warning

危险声明

预防措施声明

危险分类

Eye Irrit. 2

储存分类代码

10 - Combustible liquids

闪点(°F)

Not applicable

闪点(°C)

Not applicable


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H Hidaka et al.
Journal of lipid research, 40(6), 1131-1139 (1999-06-05)
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Y Kobayashi et al.
Molecular human reproduction, 3(2), 91-99 (1997-02-01)
Leiomyomas are tumours of uterine smooth muscle tissue that are oestrogen and progesterone dependent. When explants of these tumours were grown in culture, the proliferating tissue formed characteristic ball-like aggregates (BLA), rather than the usual hill and valley (HV) pattern

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