推荐产品
用途
kit sufficient for 50 reactions
品質等級
分析物化學類別
proteins (FLAG® Affinity Gels)
技術
immunoprecipitation (IP): suitable
運輸包裝
wet ice
儲存溫度
−20°C
一般說明
Sigma的FLAG免疫沉淀试剂盒可快速、高效地免疫沉淀和洗脱FLAG标记蛋白。免疫沉淀过程使用ANTI-FLAG M2亲和凝胶进行,它是一种与琼脂糖树脂共价连接的高特异性单克隆抗体。亲和树脂可使FLAG标记蛋白的结合高效进行,而无需预备步骤或校准。免疫沉淀后的FLAG标记蛋白可以在酸性条件下或使用竞争性FLAG肽,从树脂上高效地洗脱下来。免疫沉淀后的蛋白可用于电泳或其它活性检测方法,以检测它们的大小、翻译后修饰及相互作用。
應用
更多产品信息,请访问我们的FLAG®应用页面。
特點和優勢
- 使用高特异性ANTI-FLAG M2亲和凝胶
- 无预备步骤或校准
- 3X FLAG™肽通过直接竞争实现高效和温和的洗脱
其他說明
所有步骤在2-8°C进行。
法律資訊
3xFLAG is a trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
訊號詞
Warning
危險聲明
危險分類
Aquatic Chronic 3 - Eye Irrit. 2
儲存類別代碼
10 - Combustible liquids
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