Merck
All Photos(3)

G0799

Sigma-Aldrich

β-葡萄糖醛酸酶 来源于大肠杆菌

>20,000,000 units/g protein, recombinant, expressed in E. coli, aqueous glycerol solution

Synonym(s):
β-葡萄糖醛酸苷酶
CAS Number:
Enzyme Commission number:
EC 号:
MDL编号:
NACRES:
NA.54

质量水平

200

重组

expressed in E. coli

形式

aqueous glycerol solution

specific activity

>20,000,000 units/g protein

分子量

74 kDa

浓度

>1 mg/mL

运输

wet ice

储存温度

−20°C

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Related Categories

一般描述

β-Glucuronidase is a tetramer belonging to the glycosidase family with a molecular weight of 29 kDa. It is composed of 74 kDa identical monomers. The pI and optimal pH of the enzyme is 4.8 and 6-7 respectively.

应用

β-Glucuronidase was used in the study of Cer-β-glucuronide synthesis and evaluation of β-glucuronidase(s) from Escherichia coli or intestinal segments to release the head group.
在尿液代谢物分析中用于 葡萄糖苷酸缀合物的水解
比较不同酶性能的新技术文章
了解更多
关于Sigma R&D产生的最新应用数据,以优化不同来源的酶对不同药物类别的水解条件,并使用色谱纯化酶降低酯酶活性的影响,从而将6-MAM转化为吗啡
有效水解类固醇葡糖苷酸。

包装

25000, 100000 units in poly bottle

单位定义

One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at the pH 6.8 (30 min assay).

外形

β-glucuronidase is used as a reporter gene in GUS assays to monitor gene expression.
Highly purified solution in 50% glycerol

象形图

Health hazard

警示用语:

Danger

危险声明

预防措施声明

危险分类

Resp. Sens. 1 - Skin Sens. 1

储存分类代码

13 - Non Combustible Solids

WGK

WGK 3

闪点(F)

Not applicable

闪点(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

More Documents

Quotes and Ordering

Protocols

Using UHPLC/MS (TOF) for Detection of Drugs and Metabolites in Urine Following Optimized Enzymatic Hydrolysis Conditions

To optimize hydrolysis using β-glucuronidase, factors such as incubation time, temperature, hydrolysis pH, enzyme source, and enzyme concentration must be evaluated for each glucuronide metabolite to be analyzed.

Related Content

使用UHPLC/MS(TOF)检测不同酶水解条件处理的尿液中药物和代谢物

为了使用β-葡糖醛酸酶优化水解,必须对每种待分析的葡糖苷酸代谢物进行某些因素的评估,例如孵育时间、温度、水解pH、酶源和酶浓度。

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