G1270

L-谷氨酰胺合成酶 来源于大肠杆菌

lyophilized powder, 400-2,000 units/mg protein

• Synonym(s): L-Glutamate:ammonia ligase (ADP-forming)
• CAS Number: 9023-70-5
• Enzyme Commission number: 6.3.1.2 (BRENDA, IUBMB)
• MDL编号: MFCD00131463
• NACRES: NA.26

Properties

• 形式: lyophilized powder
• 质量水平: 200
• specific activity: 400-2,000 units/mg protein
• 纯化方式: affinity chromatography
• 包含: dithioerythritol as preservative
• 组成: Protein, ~5% Lowry
• 溶解性: H₂O: soluble 0.95-1.05 mg/mL, clear to hazy
• UniProt登记号: P0A9C5
• 异质活性: ATPase <0.2%
• 储存温度: −20°C
• Gene Information: Escherichia coli K12 ... glnA(948370)

Description

一般描述

L-Glutamine Synthetase from bacteria shows dodecameric structure comprising of 12 active sites. Each active site termed bifunnel, has an ATP and glutamate binding sites. The dodecamer is stabilized by two hexameric rings.

应用

L-Glutamine Synthetase from Escherichia coli has been used in the synthesis of methylglutamine from methylammonium in E coli and in the glutamine synthetase protection activity of human thioredoxin peroxidase enzyme, AOE372.

L-Glutamine synthetase may be used for the purification of proteases from Escherichia coli.

生化/生理作用

L-glutamine synthetase catalyzes the condensation of L-glutamate and ammonia to L-glutamine. It is a degradative enzyme for glutamic acid.

Nitrogen starvation dictates the expression of the glutamine synthetase (GS) gene in E. coli. GS plays a key role in ammonia assimilation in bacteria. Adenylylation of GS is catalyzed by adenylyltransferase. Adenylylation of GS modulates its catalytic functionality resulting in glutamine limitation in E coli.

谷氨酸降解酶

单位定义

One unit will convert 1.0 μmole of L-glutamate to L-glutamine in 15 min at pH 7.1 at 37 °C.

外形

Contains potassium phosphate, sodium citrate and magnesium acetate buffer salts

Safety Information

• 储存分类代码: 11 - Combustible Solids
• WGK: WGK 3
• 闪点(F): Not applicable
• 闪点(C): Not applicable
• 个人防护装备: Eyeshields, Gloves, type N95 (US)