Merck

G4134

Sigma-Aldrich

葡萄糖-6-磷酸脱氢酶 来源于面包酵母(酿酒酵母

Type IX, lyophilized powder, 200-400 units/mg protein (modified Warburg-Christian)

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别名:
6-磷酸葡糖脱氢酶, G-6-P-DH
CAS号:
EC 号:
MDL编号:
NACRES:
NA.54

类型

Type IX

质量水平

形式

lyophilized powder

specific activity

200-400 units/mg protein (modified Warburg-Christian)

分子量

128 kDa

β-NADP和β-NADPH含量

≤10 mmol/mol

application(s)

agriculture

运输

dry ice

储存温度

−20°C

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G6378G5885G8404
mol wt

128 kDa

mol wt

128 kDa

mol wt

128 kDa

mol wt

128 kDa

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

ammonium sulfate suspension

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

shipped in

dry ice

shipped in

dry ice

shipped in

-

shipped in

-

Quality Level

200

Quality Level

300

Quality Level

300

Quality Level

300

一般描述

Research area: Cell Signaling

Glucose-6-phosphate dehydrogenase (G6PD) is a key metabolic enzyme of the pentose phosphate pathway. In S. cerevisiae, it is encoded by the ZWF1 gene. G6PD exists as a tetramer in its active form.

应用

葡萄糖-6-磷酸脱氢酶用于测试玉米胚乳发育过程中的酮糖还原酶活性。它也可用于β-NADP和β-NADPH的回收微量测定。

生化/生理作用

葡萄糖-6-磷酸脱氢酶催化葡萄糖-6-磷酸转化为6-磷酸葡萄糖酸,作为磷酸戊糖途径的第一步。
Glucose-6-phosphate dehydrogenase catalyzes the rate-limiting step in the pentose phosphate pathway. Its function involves the conversion of glucose-6-phosphate to 6-phosphogluconolacetone while generating NADPH, which is essential for the regeneration of glutathione The glutathione system utilizes nicotinamide adenine dinucleotide phosphate hydrogen (NADPH) to effectively eliminate excess hydrogen peroxide. Glucose-6-phosphate dehydrogenase (G6PD) plays an important role in regulating cell growth and survival. Their levels are higher in cells undergoing normal and neoplastic growth. Increased glucose-6-phosphate dehydrogenase activity plays a pivotal role in preventing reactive oxygen species mediated cell death. Glucose-6-phosphate dehydrogenase is over expressed in several cancers whereas its activity is reduced in hyperglycemia. A deficiency in glucose-6-phosphate dehydrogenase causes hemolysis.

单位定义

一个酶活性单位是指在pH 7.4、25°C且存在NADP的条件下,每分钟将1.0 μM的 D-葡萄糖6-磷酸氧化成6-磷酸-D-葡糖酸盐所需的酶量。

外形

冻干粉,基本上无硫酸盐,含有大约20% 的柠檬酸钠

象形图

Health hazard

警示用语:

Danger

危险声明

预防措施声明

危险分类

Resp. Sens. 1

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)


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The global prevalence of glucose-6-phosphate dehydrogenase deficiency: A systematic review and meta-analysis
Nkhoma ET and Poole C
Blood Cells, Molecules and Diseases, 42, 267?278-267?278 (2009)
The Pentose Phosphate Pathway in Yeasts-More Than a Poor Cousin of Glycolysis
Bertels LK, et al.
Biomolecules, 11(5), 725-725 (2021)
Glucose-6-phosphate dehydrogenase, NADPH, and cell survival
Stanton R C
IUBMB Life, 64(5), 362-369 (2012)
Impact of glucose-6-phosphate dehydrogenase deficiency on the pathophysiology of cardiovascular disease
Hecker PA, et al.
American Journal of Physiology. Heart and Circulatory Physiology, 304(4), H491-H500 (2013)
D Oh et al.
Molecular and cellular biology, 10(4), 1415-1422 (1990-04-01)
The Saccharomyces cerevisiae GAL5 (PGM2) gene was isolated and shown to encode the major isozyme of phosphoglucomutase. Northern (RNA) blot hybridization revealed that the GAL5 transcript level increased three- to fourfold in response to galactose and was severely repressed in

实验方案

To measure hexokinase activity, a continuous spectrophotometric rate-determination assay is used at 340 nm. Hexokinase catalyzes the phosphorylation of D-hexose sugars using ATP as the phosphate source.

在测定己糖激酶活性时,使用了一种在340nm处的连续分光光度法进行测定。己糖激酶使用ATP作为磷酸的来源来催化D-己糖的磷酸化。

To measure glucose-6-phosphate dehydrogenase activity, beta-nicotinamide adenine dinucleotide phosphate is used in a spectrophotometric rate determination assay at 340 nm.

在测定6-磷酸葡萄糖脱氢酶活性时,使用β-烟酰胺腺嘌呤二核苷酸磷酸在340 nm处通过分光光度法进行测定。

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