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Merck

G4913

Sigma-Aldrich

Anti-Glutamic Acid Decarboxylase 65 (514-530) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

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别名:
Anti-GAD 65
MDL號碼:
分類程式碼代碼:
12352203
NACRES:
NA.43

生物源

rabbit

共軛

unconjugated

抗體表格

IgG fraction of antiserum

抗體產品種類

primary antibodies

無性繁殖

polyclonal

形狀

buffered aqueous solution

物種活性

mouse, pig, human, rat

技術

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500 using tissue sections of rat pancreas
microarray: suitable
western blot: 1:4,000 using rat brain extract

UniProt登錄號

運輸包裝

dry ice

儲存溫度

−20°C

目標翻譯後修改

unmodified

基因資訊

human ... GAD2(2572)
mouse ... Gad2(14417)
rat ... Gad2(24380)

相关类别

一般說明

GAD is predominantly expressed in the central nervous system (CNS) and pancreatic islets. It is also expressed in testis, oviduct and ovary. GAD exists in two isofoms, GAD 65 and GAD 67. GAD65 is an ampiphilic, membrane-anchored protein, (585 amino acid residues) and is encoded on human chromosome 10.

特異性

Reacts specifically with GAD 65 (65 kDa) from brain.

免疫原

synthetic peptide corresponding to the C-terminal region of human GAD 65 (amino acids 514-530). The sequence is identical in mouse GAD 65 and highly conserved in rat and pig GAD 65 but not found in GAD 67.

應用

Anti-Glutamic Acid Decarboxylase 65 antibody produced in rabbit is suitable as a primary antibody for the determination of expression of GAD 65 isoform in putative P boutons in rat and mouse at a working dilution of 1:5000 and in lysates prepared from rat testis at different developmental ages and rat cerebellum by immunoblotting.
It is also suitable for immunohistochemistry at a working dilution of 1:500 using formalin-fixed, paraffin-embedded sections of rat pancreas and for microarray.

生化/生理作用

Glutamic Acid Decarboxylase (GAD) catalyzes the conversion of L-glutamate to γ-aminobutyric acid (GABA), the principal inhibitory neurotransmitter in the brain, and a putative paracrine signal molecule in pancreatic islets. It has been identified as an autoantigen in insulin-dependent diabetes mellitus (IDDM) and stiff-man syndrome (SMS) and may serve as a marker in the early stages of IDDM. GAD 65 synthesizes transmitter GABA for vesicular release.

外觀

0.01M 磷酸缓冲盐溶液,pH 7.4,含 15mM 叠氮化钠。

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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Haixiong Liu et al.
Acta biochimica et biophysica Sinica, 41(7), 545-553 (2009-07-07)
Glutamate decarboxylase 65 (GAD65) produces gamma-aminobutyric acid, the main inhibitory neurotransmitter in adult mammalian brain. Previous experiments, performed in brain, showed that GAD65 gene possesses two TATA-less promoters, although the significance is unknown. Here, by rapid amplification of cDNA ends
J J Soghomonian et al.
Trends in pharmacological sciences, 19(12), 500-505 (1999-01-01)
Adults express two isoforms of glutamate decarboxylase (GAD), GAD67 and GAD65, which are encoded by different independently regulated genes, a situation that differs from that of other neurotransmitters. In this article, J-J. Soghomonian and David Martin review current knowledge on
A Reetz et al.
The EMBO journal, 10(5), 1275-1284 (1991-05-01)
GABA, a major inhibitory neurotransmitter of the brain, is also present at high concentration in pancreatic islets. Current evidence suggests that within islets GABA is secreted from beta-cells and regulates the function of mantle cells (alpha- and delta-cells). In the
S Baekkeskov et al.
Nature, 347(6289), 151-156 (1990-09-13)
The pancreatic islet beta-cell autoantigen of relative molecular mass 64,000 (64K), which is a major target of autoantibodies associated with the development of insulin-dependent diabetes mellitus (IDDM) has been identified as glutamic acid decarboxylase, the biosynthesizing enzyme of the inhibitory
K Kucharova et al.
Cell transplantation, 20(8), 1163-1178 (2011-06-15)
A major limitation of neural transplantation studies is assessing the degree of host-graft interaction. In the present study, rat hippocampal/cortical embryonic neurons (E18) were infected with a lentivirus encoding enhanced green fluorescent protein (GFP) under control of the neuron-specific

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