Merck

G6378

Sigma-Aldrich

葡萄糖-6-磷酸脱氢酶 来源于面包酵母(酿酒酵母

Type XV, lyophilized powder, 200-400 units/mg protein (modified Warburg-Christian)

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别名:
6-磷酸葡糖脱氢酶, G-6-P-DH
CAS号:
EC 号:
MDL编号:
NACRES:
NA.54

类型

Type XV

质量水平

形式

lyophilized powder

specific activity

200-400 units/mg protein (modified Warburg-Christian)

分子量

128 kDa

纯化方式

crystallization

application(s)

diagnostic assay manufacturing

运输

dry ice

储存温度

−20°C

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此商品
G4134G5885G8529
specific activity

200-400 units/mg protein (modified Warburg-Christian)

specific activity

200-400 units/mg protein (modified Warburg-Christian)

specific activity

>= 550 units/mg protein (biuret)

specific activity

≥550 units/mg protein (biuret)

mol wt

128 kDa

mol wt

128 kDa

mol wt

128 kDa

mol wt

-

purified by

crystallization

purified by

-

purified by

-

purified by

-

application(s)

diagnostic assay manufacturing

application(s)

agriculture

application(s)

agriculture

application(s)

agriculture

shipped in

dry ice

shipped in

dry ice

shipped in

-

shipped in

-

一般描述

葡萄糖6-磷酸脱氢酶(G-6-P-DH)是磷酸戊糖途径第一步的关键调节酶。G-6-P-DH是分子量为128kDa(凝胶过滤)的糖蛋白

应用

葡萄糖-6-磷酸脱氢酶被用于测试玉米胚乳形成中的酮糖还原酶活性。

生化/生理作用

葡萄糖-6-磷酸脱氢酶催化葡萄糖-6-磷酸转化为6-磷酸葡萄糖酸,作为磷酸戊糖途径的第一步<<<17>>>。
葡萄糖-6-磷酸脱氢酶催化葡萄糖-6-磷酸转化为6-磷酸葡萄糖酸,作为磷酸戊糖途径的第一步。

单位定义

一个酶活性单位是指在pH 7.4、25°C且存在NADP的条件下,每分钟将1.0 μM的 D-葡萄糖6-磷酸氧化成6-磷酸-D-葡糖酸盐所需的酶量。

外形

冻干粉,基本上无硫酸盐,按干重计含有大约20% 的柠檬酸钠

制备说明

由 G7877 制备

象形图

Health hazard

警示用语:

Danger

危险声明

预防措施声明

危险分类

Resp. Sens. 1

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)


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K E Reilly et al.
Biochemical and biophysical research communications, 216(3), 993-998 (1995-11-22)
A commercial preparation of glucose-6-phosphate dehydrogenase (G6PD) purified from Saccharomyces cerevisiae was subjected to PAGE analysis under both nondenaturing and denaturing conditions. The enzyme, identified by both activity staining and anti-yeast G6PD antibody immunoblotting, was shown to contain carbohydrate using
D C Doehlert
Plant physiology, 84(3), 830-834 (1987-07-01)
Ketose reductase (NAD-dependent polyol dehydrogenase EC 1.1.1.14) activity, which catalyzes the NADH-dependent reduction of fructose to sorbitol (d-glucitol), was detected in developing maize (Zea mays L.) endosperm, purified 104-fold from this tissue, and partially characterized. Product analysis by high performance
Yong Yang et al.
Frontiers in microbiology, 9, 1428-1428 (2018-07-20)
Mycobacteria spontaneously form surface-associated multicellular communities, called biofilms, which display resistance to a wide range of exogenous stresses. A causal relationship between biofilm formation and emergence of stress resistance is not known. Here, we report that activation of a nitrogen
P Andrews
The Biochemical journal, 96(3), 595-606 (1965-09-01)
1. Correlation between elution volume, V(e), and molecular weight was investigated for gel filtration of proteins of molecular weights ranging from 3500 (glucagon) to 820000 (alpha-crystallin) on Sephadex G-200 columns at pH7.5. 2. Allowing for uncertainties in the molecular weights
María J Rodríguez-López et al.
Insects, 11(9) (2020-09-13)
A combination of biological control and host plant resistance would be desirable for optimally controlling the greenhouse whitefly, Trialeurodes vaporariorum in tomato crops. Whitefly settlement preference, oviposition, and survivorship were evaluated on ABL 10-4 and 'Moneymaker', two nearly-isogenic tomato lines

实验方案

To measure glucose-6-phosphate dehydrogenase activity, beta-nicotinamide adenine dinucleotide phosphate is used in a spectrophotometric rate determination assay at 340 nm.

在测定6-磷酸葡萄糖脱氢酶活性时,使用β-烟酰胺腺嘌呤二核苷酸磷酸在340 nm处通过分光光度法进行测定。

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