Merck
All Photos(1)

G6392

Sigma-Aldrich

L-谷氨酰胺

γ-irradiated, BioXtra, suitable for cell culture

Synonym(s):
(S)-2,5-二氨基-5-氧代戊酸, L-谷氨酸-5-酰胺, 左谷酰胺
Linear Formula:
H2NCOCH2CH2CH(NH2)CO2H
CAS Number:
Molecular Weight:
146.14
Beilstein:
1723797
EC 号:
MDL编号:
eCl@ss:
32160406
PubChem化学物质编号:
NACRES:
NA.75

质量水平

无菌性

γ-irradiated

产品线

BioXtra

形式

powder

包装

vial of 0.292 g

technique(s)

cell culture | mammalian: suitable

杂质

endotoxin, tested

颜色

white to faint yellow

mp

185 °C (dec.) (lit.)

溶解性

H2O: 10 mL/vial, clear to slightly hazy

SMILES string

N[C@@H](CCC(N)=O)C(O)=O

InChI

1S/C5H10N2O3/c6-3(5(9)10)1-2-4(7)8/h3H,1-2,6H2,(H2,7,8)(H,9,10)/t3-/m0/s1

InChI key

ZDXPYRJPNDTMRX-VKHMYHEASA-N

Gene Information

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L-Glutamine γ-irradiated, BioXtra, suitable for cell culture

Sigma-Aldrich

G6392

L-谷氨酰胺

L-Glutamine ReagentPlus®, ≥99% (HPLC)

Sigma-Aldrich

G3126

L-谷氨酰胺

L-Glutamine meets USP testing specifications, suitable for cell culture, 99.0-101.0%, from non-animal source

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G8540

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L-Glutamine for biochemistry

Millipore

1.00289

L-谷氨酰胺

product line

BioXtra

product line

ReagentPlus®

product line

-

product line

-

form

powder

form

powder

form

powder

form

solid

technique(s)

cell culture | mammalian: suitable

technique(s)

-

technique(s)

cell culture | mammalian: suitable

technique(s)

-

impurities

endotoxin, tested

impurities

≤0.2% free ammonia., ~0.5% Glutamic acid

impurities

endotoxin, tested

impurities

-

color

white to faint yellow

color

white to off-white

color

white

color

-

mp

185 °C (dec.) (lit.)

mp

185 °C (dec.) (lit.)

mp

185 °C (dec.) (lit.)

mp

185 °C (dec.) (lit.)

应用

L-谷氨酰胺已作为培养基补充剂用于:
  • 耳蜗器官型培养
  • Iscove′s改良Dulbecco′s培养基中,以进行HeLa细胞培养
  • Dulbecco′s改良Eagle′s培养基中,以进行肝细胞原代培养

生化/生理作用

L-谷氨酰胺是一种必需氨基酸,是培养基的重要成分,也是培养细胞的主要能源。最佳的细胞性能通常需要在使用前添加 L-谷氨酰胺的培养基。
谷氨酰胺通过细胞mTOR(雷帕霉素的哺乳动物靶标)信号转导介导蛋白质的周转。其还已报到可与细胞凋亡的抑制有关。
L-谷氨酰胺是一种必需氨基酸,作为细胞培养过程中主要的能量来源而成为培养基的重要组分。L-谷氨酰胺在干粉及冷冻溶液的状态下极为稳定。然而,L-谷氨酰胺在液体培养基或储存溶液中均会相对较快的降解。最佳的细胞表现通常需要在使用前向培养基中添加L-谷氨酰胺补充剂。

包装

带橡胶塞的 50 mL 琥珀色血清瓶。

适用性

适用于细胞培养应用,尤其是无血清细胞培养。

重悬

用无菌水复溶至 10 mL 时,制备 200 mM 溶液。

其他说明

二肽 L-Ala-LGln 经常用于现代无血清培养基配方中,因为它在体外提供了更稳定的谷氨酰胺形式。

储存分类代码

11 - Combustible Solids

WGK

WGK 1

闪点(F)

Not applicable

闪点(C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)


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