G6392

Sigma-Aldrich

L-谷氨酰胺

γ-irradiated, BioXtra, suitable for cell culture

别名:
L-谷氨酸-5-酰胺, 左谷酰胺, (S)-2,5-二氨基-5-氧代戊酸
线性分子式:
H2NCOCH2CH2CH(NH2)CO2H
CAS号:
分子量:
146.14
Beilstein/REAXYS Number:
1723797
EC 号:
MDL编号:
eCl@ss:
32160406
PubChem化学物质编号:
NACRES:
NA.75

质量水平

200

无菌性

γ-irradiated

产品线

BioXtra

形式

powder

包装

vial of 0.292 g

应用

cell culture | mammalian: suitable

杂质

endotoxin, tested

颜色

white to faint yellow

mp

185 °C (dec.) (lit.)

溶解性

H2O: 10 mL/vial, clear to slightly hazy

SMILES string

N[C@@H](CCC(N)=O)C(O)=O

InChI

1S/C5H10N2O3/c6-3(5(9)10)1-2-4(7)8/h3H,1-2,6H2,(H2,7,8)(H,9,10)/t3-/m0/s1

InChI key

ZDXPYRJPNDTMRX-VKHMYHEASA-N

Gene Information

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相关类别

应用

L-谷氨酰胺已作为培养基补充剂用于:
  • 耳蜗器官型培养
  • Iscove′s改良Dulbecco′s培养基中,以进行HeLa细胞培养
  • Dulbecco′s改良Eagle′s培养基中,以进行肝细胞原代培养

生化/生理作用

L-谷氨酰胺是一种必需氨基酸,是培养基的重要成分,也是培养细胞的主要能源。最佳的细胞性能通常需要在使用前添加 L-谷氨酰胺的培养基。
谷氨酰胺通过细胞mTOR(雷帕霉素的哺乳动物靶标)信号转导介导蛋白质的周转。其还已报到可与细胞凋亡的抑制有关。
L-谷氨酰胺是一种必需氨基酸,作为细胞培养过程中主要的能量来源而成为培养基的重要组分。L-谷氨酰胺在干粉及冷冻溶液的状态下极为稳定。然而,L-谷氨酰胺在液体培养基或储存溶液中均会相对较快的降解。最佳的细胞表现通常需要在使用前向培养基中添加L-谷氨酰胺补充剂。

包装

带橡胶塞的 50 mL 琥珀色血清瓶。

适用性

适用于细胞培养应用,尤其是无血清细胞培养。

重悬

用无菌水复溶至 10 mL 时,制备 200 mM 溶液。

其他说明

二肽 L-Ala-LGln 经常用于现代无血清培养基配方中,因为它在体外提供了更稳定的谷氨酰胺形式。

storage_class_code

13 - Non Combustible Solids

WGK Germany

WGK 1

闪点(F)

Not applicable

闪点(C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

分析证书

原产地证书 (CofO)

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. Is the molecular weight of L-Glutamine 146.14 or 146.15? The label information changed.

    Based on a molecular formula of C5H10N2O3, the molecular weight to seven significant figures is 146.1456. Over the years, this value may have been reported in Sigma-Aldrich documentation or on labels as either 146.15 (by rounding up) or 146.14 (by truncation). The material itself has not changed.

  6. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

  7. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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