文件

G6392

L-谷氨酰胺

γ-irradiated, BioXtra, suitable for cell culture

所有图片(1)

别名:

L-谷氨酸-5-酰胺, 左谷酰胺, (S)-2,5-二氨基-5-氧代戊酸

线性分子式:

H₂NCOCH₂CH₂CH(NH₂)CO₂H

CAS号:

56-85-9

分子量:

146.14

Beilstein/REAXYS Number:

1723797

EC 号:

200-292-1

MDL编号:

MFCD00008044

eCl@ss:

32160406

PubChem化学物质编号:

24895254

NACRES:

NA.75

质量水平

200

无菌性

γ-irradiated

产品线

BioXtra

形式

powder

包装

vial of 0.292 g

应用

cell culture | mammalian: suitable

杂质

endotoxin, tested

颜色

white to faint yellow

mp

185 °C (dec.) (lit.)

溶解性

H₂O: 10 mL/vial, clear to slightly hazy

SMILES string

N[C@@H](CCC(N)=O)C(O)=O

InChI

1S/C5H10N2O3/c6-3(5(9)10)1-2-4(7)8/h3H,1-2,6H2,(H2,7,8)(H,9,10)/t3-/m0/s1

InChI key

ZDXPYRJPNDTMRX-VKHMYHEASA-N

Gene Information

rat ... Ggt1(116568)

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应用

L-谷氨酰胺已作为培养基补充剂用于：

耳蜗器官型培养
Iscove′s改良Dulbecco′s培养基中，以进行HeLa细胞培养
Dulbecco′s改良Eagle′s培养基中，以进行肝细胞原代培养

生化/生理作用

L-谷氨酰胺是一种必需氨基酸，是培养基的重要成分，也是培养细胞的主要能源。最佳的细胞性能通常需要在使用前添加 L-谷氨酰胺的培养基。

谷氨酰胺通过细胞mTOR（雷帕霉素的哺乳动物靶标）信号转导介导蛋白质的周转。其还已报到可与细胞凋亡的抑制有关。

L-谷氨酰胺是一种必需氨基酸，作为细胞培养过程中主要的能量来源而成为培养基的重要组分。L-谷氨酰胺在干粉及冷冻溶液的状态下极为稳定。然而，L-谷氨酰胺在液体培养基或储存溶液中均会相对较快的降解。最佳的细胞表现通常需要在使用前向培养基中添加L-谷氨酰胺补充剂。

包装

带橡胶塞的 50 mL 琥珀色血清瓶。

适用性

适用于细胞培养应用，尤其是无血清细胞培养。

重悬

用无菌水复溶至 10 mL 时，制备 200 mM 溶液。

其他说明

二肽 L-Ala-LGln 经常用于现代无血清培养基配方中，因为它在体外提供了更稳定的谷氨酰胺形式。

storage_class_code

13 - Non Combustible Solids

WGK Germany

WGK 1

闪点(F)

Not applicable

闪点(C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

分析证书

原产地证书 (CofO)

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Is the molecular weight of L-Glutamine 146.14 or 146.15? The label information changed.
Based on a molecular formula of C5H10N2O3, the molecular weight to seven significant figures is 146.1456. Over the years, this value may have been reported in Sigma-Aldrich documentation or on labels as either 146.15 (by rounding up) or 146.14 (by truncation). The material itself has not changed.
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