所有图片(3)

G7017

Sigma-Aldrich

β-葡萄糖醛酸酶 来源于罗曼蜗牛

Type HP-2, aqueous solution, ≥100,000 units/mL

别名:
β-葡萄糖醛酸苷酶
CAS号:
MDL编号:
NACRES:
NA.54

质量水平

300

类型

Type HP-2

形式

aqueous solution

specific activity

≥100,000 units/mL

secondary activity

≤7,500 units/mL sulfatase

运输

wet ice

储存温度

2-8°C

正在寻找类似产品? Visit 产品对比指南

一般描述

β-来自玛瑙螺的H-2型葡糖醛酸糖苷酶是从罗马蜗牛的消化液中提取的酶的粗溶液。β-来自软体动物的葡糖醛酸糖苷酶也有硫酸酯酶活性。两种活性都可用于代谢物的水解,用于毒理学和生物标志物分析。如需更广泛地了解我们的β-葡糖醛酸糖苷酶产品线及其用途,请参阅我们有关药物代谢物分析的页面

应用

  • 通过消化与葡萄糖醛酸苷结合的化合物而制备的LC / MS样品,用于生物标志物和毒理学研究。
  • 去除葡萄糖醛酸苷结合物,例如用于制备黄芩素。
比较不同酶性能的新技术文章
了解更多
关于Sigma R&D产生的最新应用数据,以优化不同来源的酶对不同药物类别的水解条件,并使用色谱纯化酶降低酯酶活性的影响,从而将6-MAM转化为吗啡

包装

25 mL in glass bottle

生化/生理作用

在尿液代谢物分析中用于 葡萄糖苷酸缀合物的水解
内源性β-葡糖醛酸糖苷酶是一种溶酶体酶,参与了葡萄糖醛酸苷修饰的分解代谢产物的消化。
在微生物群中发现的微生物β-葡糖醛酸糖苷酶可以通过水解异种生物质和内生代谢物的葡萄糖醛酸苷结合物而引起代谢物的重吸收。

特点和优势

  • 最适pH 4.0 - 5.0
  • 硫酸酯酶最适pH 6.2
  • 硫酸酯酶活性低(<7,500单位/ mL)。
  • 硫酸铵悬浮液。
  • 来自软体动物的β-葡糖醛酸糖苷酶。
  • 可用于低pH样品中葡萄糖醛酸苷的酸水解。

质量

来自软体动物的多种β-葡糖醛酸糖苷酶也含有硫酸酯酶活性。

联系

与G0876相似,只是由Sigma生产的。

单位定义

在pH 5.0、温度37°C条件下,一单元sigma或改良的Fishman单元1小时会从酚酞葡糖苷酸释放 1.0 μg酚酞(30分钟实验)。
硫酸酯酶单元定义:一单位的硫酸酯酶每小时,pH5.0,37℃能够水解1.0 μmole p-硝基儿茶酚硫酸盐。

外形

在~1.0 M硫酸铵中含有3 mM叠氮化钠作为保存剂的水相溶液。

象形图

Health hazard

警示用语:

Danger

危险声明

危险分类

Resp. Sens. 1 - Skin Sens. 1

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 3

闪点(F)

Not applicable

闪点(C)

Not applicable

分析证书

原产地证书 (CofO)

Madalina Neacsu et al.
Molecular nutrition & food research, 61(3) (2016-07-01)
Phytophenols present in cereals are metabolised to compounds that could be partly responsible for the reduced risk of chronic diseases and all-cause mortality associated with fibre-rich diets. The bioavailability, form and in vivo concentrations of these metabolites require to be
Monica L Vermillion Maier et al.
Drug metabolism and disposition: the biological fate of chemicals, 49(8), 694-705 (2021-05-27)
3,3'-Diindolylmethane (DIM), a major phytochemical derived from ingestion of cruciferous vegetables, is also a dietary supplement. In preclinical models, DIM is an effective cancer chemopreventive agent and has been studied in a number of clinical trials. Previous pharmacokinetic studies in
Madalina Neacsu et al.
Foods (Basel, Switzerland), 9(4) (2020-04-09)
Legumes are a source of health-promoting macro- and micronutrients, but also contain numerous phytochemicals with useful biological activities, an example of which are saponins. Epidemiological studies suggest that saponins may play a role in protection from cancer and benefit human
Jie Xing et al.
Journal of pharmaceutical and biomedical analysis, 39(3-4), 593-600 (2005-05-17)
The stability of baicalin in buffered aqueous solutions at different pHs and in biological fluids, including plasma, urine and tissue homogenates, were investigated in vitro. Structures of the degradation products of baicalin were elucidated by liquid chromatography-electrospray ion trap mass
Kenneth K C Bramwell et al.
The Journal of clinical investigation, 124(1), 311-320 (2013-12-18)
Lyme disease, caused by the spirochete Borrelia burgdorferi, is the most prevalent arthropod-borne illness in the United States and remains a clinical and social challenge. The spectrum of disease severity among infected patients suggests that host genetics contribute to pathogenic

技术文章

Evaluation of Different Enzymes on Hydrolysis Efficiencies of Glucuronide Drug Metabolites in Urine

β-glucuronidase (GUS) enzymes are utilized to hydrolyze glucuronide (gluc) drug metabolites to the parent drug, facilitating analysis by LC-MS/MS.

实验方案

Enzymatic Assay of ß-Glucuronidase (EC 3.2.1.31) from Helix Pomatia and Bovine Liver

Enzymatic Assay of ß-Glucuronidase (EC 3.2.1.31) from Helix Pomatia and Bovine Liver. This procedure applies to all b-Glucuronidase products that are derived from Helix Pomatia and Bovine Liver. It is the responsibility of trained Analytical Services laboratory personnel to follow this procedure as written.

相关内容

酶促检测罗马蜗牛和牛肝脏来源ß-葡萄糖醛酸酶(EC 3.2.1.31)

酶促检测罗马蜗牛和牛肝脏来源ß-葡萄糖醛酸酶(EC 3.2.1.31)适用于可检测罗马蜗牛和牛肝脏来源ß-葡萄糖醛酸酶的所有产品。应由经过培训的分析服务实验室人员负责按照书面规定执行此程序。

我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门