G7663

Sigma-Aldrich

Gelatin blocking buffer

for Western blotting, powder blend

NACRES:
NA.25

质量水平

100

等级

for molecular biology

形式

powder blend

储存温度

room temp

一般描述

Gelatin Blocking Buffer is a standard reagent in Western blotting procedures. It is used to block non-specific binding on the membrane, when using nylon or nitrocellulose (but not PVDF).

包装

1 L in poly bottle

应用

Suitable for use as a non-specific blocking agent for Western blots.

重悬

Dissolve entire contents of the bottle in a final volume of 1L molecular biology grade water. The resulting solution will be faint yellow in color and does not require filtering or heating before use.

storage_class_code

11 - Combustible Solids

WGK Germany

WGK 1

闪点(F)

Not applicable

闪点(C)

Not applicable

分析证书

原产地证书 (CofO)

A Fortin et al.
Biochemistry and cell biology = Biochimie et biologie cellulaire, 72(5-6), 239-243 (1994-05-01)
The major advantages of the horseradish peroxidase chemiluminescence (HRP-CL) immunodetection method in Western blot analysis are its high sensitivity, nonradioactive detection, economy of the primary antibody, and speed of detecting the signal. However, we observed a strong and reproducible signal...
A sensitive cell-based assay for the detection of residual infectious West Nile virus.
Koldijk, M.H., et al.
Vaccine, 25, 39-39 (2007)
Raul Bettencourt et al.
Comparative biochemistry and physiology. Part A, Molecular & integrative physiology, 152(2), 278-289 (2008-12-02)
The interaction between microorganisms and host defense mechanisms is a decisive factor for the survival of marine bivalves. They rely on cell-mediated and humoral reactions to overcome the pathogens that naturally occur in the marine environment. In order to understand...
Iram Liaqat et al.
Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology], 43(3), 969-980 (2012-07-01)
Enterotoxigenic Escherichia coli (ETEC) strains are leading causes of childhood diarrhea in developing countries. Adhesion is the first step in pathogenesis of ETEC infections and ETEC pili designated colonization factor antigens (CFAs) are believed to be important in the biofim...
Daisuke Tomida et al.
Investigative ophthalmology & visual science, 52(6), 3193-3199 (2011-02-08)
To study the effect of heparin on the development of laser-induced choroidal neovascularization (CNV) and to assess the underlying molecular mechanisms. Bone marrow transplantation (BMT) was conducted by intravenous injection of green fluorescence protein (GFP)-labeled bone marrow cells (1 ×...
实验方案
In order to specifically detect an antigen or target molecule immobilized on a solid support, unoccupied binding sites on the support must be blocked against binding by probe and detection molecules.
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An introduction to both Northern and Southern blotting, popular methods for the transfer of macromolecules to membranous support. This article also offers a Southern blot protocol and a northern blot protocol.
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