所有图片(9)

G8132

Sigma-Aldrich

β-葡萄糖醛酸酶 来源于帽贝 (Patella vulgata)

Type L-II, lyophilized powder, 1,000,000-3,000,000 units/g solid

别名:
β-葡萄糖醛酸苷酶
CAS号:
EC 号:
MDL编号:

质量水平

300

类型

Type L-II

形式

lyophilized powder

specific activity

1,000,000-3,000,000 units/g solid

运输

wet ice

储存温度

−20°C

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应用

已证明β-L-II 型葡糖醛酸酶是尿液中药物葡糖醛酸苷水解的优越酶。
据报道,来自欧洲帽贝的酶比来自盖罩大蜗牛、牛肝脏和大肠杆菌的酶能更有效地水解阿片肽-葡糖苷酸
比较不同酶性能的新技术文章
了解更多关于 Sigma R&D 生成的最新应用数据 使用不同来源的酶优化不同药物类别的水解,并使用色谱纯化酶降低酯酶活性的影响,导致 6-MAM 转化为吗啡

包装

100000, 250000, 500000, 1000000, 2000000 units in poly bottle

生化/生理作用

β-葡萄糖醛酸酶(β-GIc)是一种外切糖苷酶,催化复合碳水化合物的分解。在人体内可将结合胆红素转化为非结合形式,使胆红素适于重吸收。使用从 Patella vulgate 获得的 ß-葡糖苷酸酶将 3-β-D 葡糖苷酸转化为吗啡。

质量

含有被 0.1 M 磷酸盐抑制的硫酸酯酶活性。

单位定义

在37℃,pH 3.8(30分钟检测)条件下,一个Sigma或改良的Fishman单位可每小时从酚酞葡糖苷酸中释放1.0 μg酚酞。

象形图

Health hazard

警示用语:

Danger

危险声明

预防措施声明

危险分类

Resp. Sens. 1 - Skin Sens. 1

储存分类代码

13 - Non Combustible Solids

WGK

WGK 3

闪点(F)

Not applicable

闪点(C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

分析证书

原产地证书 (CofO)

Vectors with the gus reporter gene for identifying and quantitating promoter regions in <I>Saccharomyces cerevisiae</I>
S.V. Marathe &amp; J.E. McEwen
Gene, 154, 105-107 (1994)
J D McCarter et al.
Current opinion in structural biology, 4(6), 885-892 (1994-12-01)
The determination of a large number of three-dimensional structures of glycosidases, both free and in complex with ligands, has provided valuable new insights into glycosidase catalysis, especially when coupled with results from studies of specifically labelled glycosidases and kinetic analyses
J Combie et al.
Clinical chemistry, 28(1), 83-86 (1982-01-01)
beta-Glucuronidase from Patella vulgata, Helix aspersa, Helix pomatia, and bovine liver were evaluated for usefulness in routine hydrolysis of drug-glucuronic acid conjugates from equine urine samples. Factors affecting the reaction rate (enzyme concentration, ligand concentration, temperature, and pH) were optimized.
Catalytic mechanisms of enymatic glycosyl transfer
M.L. Sinnott
Chemical Reviews, 90, 1171-1202 (1990)
T A Jennison et al.
Journal of analytical toxicology, 17(4), 208-210 (1993-07-01)
Methods to confirm morphine in urine require hydrolysis to liberate morphine from its 3-beta-D glucuronide (M-3G) conjugate. Lengthy enzyme hydrolysis procedures prolong testing turnaround time whereas rapid enzyme methods may produce a low conversion of M-3G to morphine. The purpose

技术文章

Evaluation of Different Enzymes on Hydrolysis Efficiencies of Glucuronide Drug Metabolites in Urine

β-glucuronidase (GUS) enzymes are utilized to hydrolyze glucuronide (gluc) drug metabolites to the parent drug, facilitating analysis by LC-MS/MS.

实验方案

Enzymatic Assay of ß-Glucuronidase (EC 3.2.1.31) from Helix Pomatia and Bovine Liver

Enzymatic Assay of ß-Glucuronidase (EC 3.2.1.31) from Helix Pomatia and Bovine Liver. This procedure applies to all b-Glucuronidase products that are derived from Helix Pomatia and Bovine Liver. It is the responsibility of trained Analytical Services laboratory personnel to follow this procedure as written.

Using UHPLC/MS (TOF) for Detection of Drugs and Metabolites in Urine Following Optimized Enzymatic Hydrolysis Conditions

To optimize hydrolysis using β-glucuronidase, factors such as incubation time, temperature, hydrolysis pH, enzyme source, and enzyme concentration must be evaluated for each glucuronide metabolite to be analyzed.

相关内容

酶促检测罗马蜗牛和牛肝脏来源ß-葡萄糖醛酸酶(EC 3.2.1.31)

酶促检测罗马蜗牛和牛肝脏来源ß-葡萄糖醛酸酶(EC 3.2.1.31)适用于可检测罗马蜗牛和牛肝脏来源ß-葡萄糖醛酸酶的所有产品。应由经过培训的分析服务实验室人员负责按照书面规定执行此程序。

使用UHPLC/MS(TOF)检测不同酶水解条件处理的尿液中药物和代谢物

为了使用β-葡糖醛酸酶优化水解,必须对每种待分析的葡糖苷酸代谢物进行某些因素的评估,例如孵育时间、温度、水解pH、酶源和酶浓度。

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