所有图片(4)

G8295

Sigma-Aldrich

β-葡萄糖醛酸酶 来源于大肠杆菌

≥10,000,000 units/g protein (30 min assay), recombinant, expressed in E. coli overproducing strain, lyophilized powder

别名:
β-葡萄糖醛酸苷酶
CAS号:
EC 号:
MDL编号:

质量水平

300

重组

expressed in E. coli overproducing strain

形式

lyophilized powder

specific activity

≥10,000,000 units/g protein (30 min assay)

分子量

~290 kDa

组成

protein, 40-60% biuret

储存温度

−20°C

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包装

25000, 500000, 2000000 units in glass bottle

应用

在尿液代谢物分析中用于 葡萄糖苷酸缀合物的水解
比较不同酶性能的新技术文章
了解更多
关于Sigma R&D产生的最新应用数据,以优化不同来源的酶对不同药物类别的水解条件,并使用色谱纯化酶降低酯酶活性的影响,从而将6-MAM转化为吗啡
有效水解类固醇葡糖苷酸。

质量

含有缓冲盐和稳定剂。

单位定义

一个Sigma或改良的Fishman单元将在37℃,pH6.8(30分钟测定)下每小时从酚酞葡糖苷酸中释放1.0 μg酚酞。

象形图

Health hazard

警示用语:

Danger

危险声明

预防措施声明

危险分类

Resp. Sens. 1 - Skin Sens. 1

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(F)

Not applicable

闪点(C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

分析证书

原产地证书 (CofO)

Enzymes
Methods of Enzymatic Analysis, 2, 460-461 (1974)
Pascale Fança-Berthon et al.
The Journal of nutrition, 151(7), 1802-1816 (2021-04-21)
Curcuminoids from turmeric rhizome have significant health benefits but low bioavailability. To assess the pharmacokinetics of a novel natural turmeric dried colloidal suspension compared with 4 other turmeric formulations (including a standardized extract) at their respective recommended dosages. Thirty healthy men
Optimized conditions for the emzymatic hydrolysis of a-hydroxytriazolam-glucoronide in human urine.
Tsujikawa, K., et al.
Health Laboratory Science, 50, 286-289 (2004)
V Graef et al.
Clinical chemistry, 23(3), 532-535 (1977-03-01)
We determined the enzymic activity of beta-glucuronidase preparations from bovine liver, Helix pomatia, and Escherischia coli with steroid glucuronides and nonsteroid glucuronides as substrates. We also studied the effect of Na2SO4 on the enzymic hydrolysis of several substrates with the
Kenneth K C Bramwell et al.
The Journal of clinical investigation, 124(1), 311-320 (2013-12-18)
Lyme disease, caused by the spirochete Borrelia burgdorferi, is the most prevalent arthropod-borne illness in the United States and remains a clinical and social challenge. The spectrum of disease severity among infected patients suggests that host genetics contribute to pathogenic

技术文章

Evaluation of Different Enzymes on Hydrolysis Efficiencies of Glucuronide Drug Metabolites in Urine

β-glucuronidase (GUS) enzymes are utilized to hydrolyze glucuronide (gluc) drug metabolites to the parent drug, facilitating analysis by LC-MS/MS.

Probiotics and Human Health

Today, diverse studies report the benefits of probiotics, such as inhibitory effects on pathogens, aid in the management or prevention of chronic intestinal inflammatory diseases or atopic syndromes, and support to the immune system. Potential beneficial applications abound, researchers continue to evaluate the effictiveness and clarify the mechanisms of action of probiotics.

实验方案

Enzymatic Assay of β-Glucuronidase (EC 3.2.1.31) from E. coli

Objective: To standardize a procedure for the enzymatic assay of β-Glucuronidase.

Using UHPLC/MS (TOF) for Detection of Drugs and Metabolites in Urine Following Optimized Enzymatic Hydrolysis Conditions

To optimize hydrolysis using β-glucuronidase, factors such as incubation time, temperature, hydrolysis pH, enzyme source, and enzyme concentration must be evaluated for each glucuronide metabolite to be analyzed.

相关内容

使用UHPLC/MS(TOF)检测不同酶水解条件处理的尿液中药物和代谢物

为了使用β-葡糖醛酸酶优化水解,必须对每种待分析的葡糖苷酸代谢物进行某些因素的评估,例如孵育时间、温度、水解pH、酶源和酶浓度。

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