G8540

L-谷氨酰胺

meets USP testing specifications, suitable for cell culture, 99.0-101.0%, from non-animal source

• Synonym(s): (S)-2,5-二氨基-5-氧代戊酸, L-谷氨酸-5-酰胺, 左谷酰胺
• Linear Formula: H₂NCOCH₂CH₂CH(NH₂)CO₂H
• CAS Number: 56-85-9
• Molecular Weight: 146.14
• Beilstein: 1723797
• EC 号: 200-292-1
• MDL编号: MFCD00008044
• eCl@ss: 32160406
• PubChem化学物质编号: 24895342
• NACRES: NA.26

Properties

• 生物来源: non-animal source
• 质量水平: 300
• Agency: meets USP testing specifications
• 检测方案: 99.0-101.0%
• 形式: powder
• technique(s): cell culture | mammalian: suitable
• 杂质: endotoxin, tested
• 颜色: white
• mp: 185 °C (dec.) (lit.)
• 溶解性: H₂O: 25 mg/mL
• application(s): agriculture
• SMILES string: N[C@@H](CCC(N)=O)C(O)=O
• InChI: 1S/C5H10N2O3/c6-3(5(9)10)1-2-4(7)8/h3H,1-2,6H2,(H2,7,8)(H,9,10)/t3-/m0/s1
• InChI key: ZDXPYRJPNDTMRX-VKHMYHEASA-N
• Gene Information: rat ... Ggt1(116568)

Description

一般描述

L-Glutamine is the most abundant amino acid in mammalian blood and tissues. It is vital for biochemical and cell culture research. It supports cell growth, metabolism, and protein synthesis. In cell culture, it ensures cell viability, proliferation, and antibody production. In biochemical studies, L-Glutamine aids in metabolic pathways, biomolecule synthesis, and cellular processes.

应用

L-谷氨酰胺已被用作制备无钙mCZB培养基的组分之一。它还被用作大鼠海马体切片培养基中的补充剂。

L-Glutamine amino acid has been used:

• as a component of the serum-free medium to culture mice cochlear organotypic cells.
• as a supplement in Dulbecco′s modified Eagle′s medium-nutrient mixture F-12 (DMEM/F-12) medium for culturing mice seminiferous tubule segments.
• as a supplement in Roswell Park Memorial Institute (RPMI) 1640 medium to culture human monocytic cell line.

生化/生理作用

谷氨酰胺可在细胞培养期间提供细胞生长和分裂所需的氮、碳和还原当量。L-谷氨酸可帮助将NH₄⁺插入至生物分子中。

L-Glutamine or Glutamine powder serves as a precursor for the synthesis of peptides, proteins, nucleotides, purine, pyrimidine, amino sugars, and nucleic acids. It plays an important role in promoting and maintaining cellular functions. Many cells and tissues like the intestine, kidneys, neurons in the central nervous system (CNS), liver, and pancreatic β-cells utilize glutamine at higher levels for their functioning. Glutamine regulates acid-base homeostasis and catabolic signaling. Glutamine plays a vital role in nitrogen transport and maintains the cellular redox state.Glutamine provides the necessary nitrogen, carbon and the reducing equivalents required for cell growth and division during cell culture. L-glutamic acid aids the incorporation of NH⁴⁺ into biomolecules.

L-谷氨酰胺是一种必需氨基酸，作为细胞培养过程中主要的能量来源而成为培养基的重要组分。L-谷氨酰胺在干粉及冷冻溶液的状态下极为稳定。然而，L-谷氨酰胺在液体培养基或储存溶液中均会相对较快的降解。最佳的细胞表现通常需要在使用前向培养基中添加L-谷氨酰胺补充剂。

Safety Information

• 储存分类代码: 11 - Combustible Solids
• WGK: WGK 1
• 闪点(F): Not applicable
• 闪点(C): Not applicable
• 个人防护装备: Eyeshields, Gloves, type N95 (US)