Merck
All Photos(1)

H0887

Sigma-Aldrich

羟乙基哌嗪乙硫磺酸 溶液

1 M, pH 7.0-7.6, sterile-filtered, BioReagent, suitable for cell culture

Synonym(s):
N-(2-羟乙基)哌嗪-N′-(2-乙磺酸)
CAS Number:
MDL编号:
PubChem化学物质编号:
NACRES:
NA.75

无菌性

sterile-filtered

质量水平

300

产品线

BioReagent
BioXtra

形式

liquid

浓度

1 M

technique(s)

cell culture | mammalian: suitable

杂质

endotoxin, tested

pH值(酸碱度)

7.0-7.6

useful pH range

6.8-8.2

储存温度

2-8°C

SMILES string

OCCN1CCN(CCS(=O)(O)=O)CC1

InChI

1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)

InChI key

JKMHFZQWWAIEOD-UHFFFAOYSA-N

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一般描述

HEPES被认为是生物学研究中最好的通用缓冲液之一。[1]在生物pH值下,该分子为两性离子,在pH 6.8~8.2(pKa 7.55)时起缓冲作用。它在细胞培养中常用的浓度为5mM到30mM。HEPES已被广泛应用于一系列应用中,包括组织培养。常用来缓冲空气中的细胞培养基。HEPES在Mg的 体外 实验中得到了应用。[2]

应用

HEPES已被作为组份应用于:
  • Hank平衡盐溶液,Dulbecco的改良eagle培养基和无糖DMEM,用于组织切片的制备[3][4]
  • 缓冲液用于在培养插入物的基侧进行局域电导测量,以检查阳离子选择性[4]

注意

浓度超过40mM的HEPES对某些细胞类型可能会有毒性。

制备说明

由HEPES游离酸和细胞培养级水制备。

储存分类代码

10 - Combustible liquids

WGK

WGK 1

闪点(F)

Not applicable

闪点(C)

Not applicable

Certificate of Analysis

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Certificate of Origin

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