Merck

H0887

Sigma-Aldrich

羟乙基哌嗪乙硫磺酸 溶液

1 M, pH 7.0-7.6, sterile-filtered, BioReagent, suitable for cell culture

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别名:
N-(2-羟乙基)哌嗪-N′-(2-乙磺酸)
CAS号:
MDL编号:
PubChem化学物质编号:
NACRES:
NA.75

无菌性

sterile-filtered

质量水平

产品线

BioReagent
BioXtra

形式

liquid

储存条件

(Tightly closed. Dry)

浓度

1 M

technique(s)

cell culture | mammalian: suitable

杂质

endotoxin, tested

pH值(酸碱度)

7.0-7.6

useful pH range

6.8-8.2

储存温度

2-8°C

SMILES string

OCCN1CCN(CCS(=O)(O)=O)CC1

InChI

1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)

InChI key

JKMHFZQWWAIEOD-UHFFFAOYSA-N

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此商品
H3537H3537H3375
vibrant-m

H0887

羟乙基哌嗪乙硫磺酸 溶液

Essential+ Grade
vibrant-m

H3537

羟乙基哌嗪乙硫磺酸 溶液

Premium Grade
vibrant-m

H3537

羟乙基哌嗪乙硫磺酸 溶液

Premium Grade
vibrant-m

H3375

HEPES

-
product line

BioReagent, BioXtra

product line

-

product line

-

product line

-

concentration

1 M

concentration

1 M

concentration

1 M

concentration

-

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

form

liquid

form

liquid

form

liquid

form

crystalline powder

pH

7.0-7.6

pH

5.0-6.0

pH

5.0-6.0

pH

5.0-6.5 (25 °C, 238 g/L)

Quality Level

300

Quality Level

200

Quality Level

200

Quality Level

400

product line

BioReagent, BioXtra

product line

-

product line

-

product line

-

concentration

1 M

concentration

1 M

concentration

1 M

concentration

-

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

form

liquid

form

liquid

form

liquid

form

crystalline powder

pH

7.0-7.6

pH

5.0-6.0

pH

5.0-6.0

pH

5.0-6.5 (25 °C, 238 g/L)

一般描述

1M的HEPES缓冲液(4-羟乙基哌嗪乙磺酸)经过无菌过滤处理,适于用作细胞生物学、生物化学和生物学研究中重要的生物缓冲液。HEPES是一种两性离子缓冲液,在pH 6.8-8.2范围内有效,适合丰富的研究领域。HEPES可用作细胞培养基的缓冲剂,相较Tris和磷酸盐有多项优势。尤其是它的细胞毒性更低,可在实验过程中维持最佳的细胞健康和活力。

此外,它可维持细胞生理pH,保护细胞免受呼吸引起的二氧化碳波动的影响,保证细胞稳态。这项能力更胜碳酸氢盐缓冲液(NaHCO3)。除了这些适合细胞培养的优异特性,HEPES也适于各种生物学和生物化学研究。也可用于免疫沉淀、细胞裂解、活细胞成像等技术,提供稳定受控的实验环境。功能多样、pH缓冲能力出色、与其他分子的互作力低,HEPES是多个研究领域中不可或缺的工具。
它在细胞培养中常用的浓度为5mM到30mM。HEPES已被广泛用于各种应用,包括组织培养。它通常用于缓冲与空气接触的细胞培养基。HEPES可用于Mg的 体外 实验。

应用

HEPES可作为组分:
  • 配制Hank’平衡盐溶液、Dulbecco’改良eagle’培养基和无糖DMEM,用于制备组织切片
  • 配制缓冲液,用于细胞培养小室底侧的局部电导测量,以检测阳离子选择性
  • 配制细胞培养基,提供缓冲能力维持细胞株pH
  • 配制缓冲液,用于猪髓核的脱细胞反应

特点和优势

  • 高纯度产品,适合生化和生物学研究
  • 除菌过滤处理,并通过细胞培养测试
  • 即用型溶液
  • 适用pH范围:6.8 - 8.2,pKa 7.5(25 °C)
  • 即用型溶液,无需配制,节省时间

注意

浓度超过40mM的HEPES对某些细胞类型可能会有毒性。

制备说明

由HEPES游离酸和细胞培养级水制备。

其他说明

For additional information on our range of Biochemicals, please complete this form.

储存分类代码

10 - Combustible liquids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable


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