Merck

H1387

Sigma-Aldrich

Hanks平衡盐

Modified, without phenol red and sodium bicarbonate, powder, suitable for cell culture

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别名:
HBSS
MDL编号:
NACRES:
NA.75

质量水平

形式

powder

technique(s)

cell culture | mammalian: suitable

储存温度

2-8°C

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Hanks平衡盐溶液 10x

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

-

Quality Level

500

Quality Level

500

Quality Level

500

Quality Level

400

HBSS, salt solutions

HBSS, salt solutions

HBSS, salt solutions

HBSS, salt solutions

一般描述

平衡盐溶液(BSS)用作冲洗、运输和稀释液体,同时维持细胞内和细胞外的渗透平衡。它还为细胞提供正常细胞代谢所必需的水和某些大块无机离子。BSS与碳水化合物(如葡萄糖)结合时,是细胞代谢的主要能量来源。此外,它还提供了一个缓冲系统,以维持培养基的生理pH值范围(7.2-7.6)。

应用

Hanks平衡盐已用于:
  • 用于在胰腺血管分离过程中稀释消化组织
  • 用于小鼠肠粘膜细胞裂解和超声前清洗
  • 用作浸渍液,测试从墨鱼骨提取的多(ε-聚己内酯)-涂覆羟基磷灰石支架的体外生物活性

数量

配制成每升培养基中含粉末 9.7 克。

重悬

补充 0.35 g/L 碳酸氢钠。

其他说明

加入碳酸氢钠后调节 pH 值。使用干细胞或细胞克隆的研究人员通常更喜欢不含酚红的培养基。

储存分类代码

13 - Non Combustible Solids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)


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实验方案

粉末培养基制备说明

粉末培养基及盐混合物极具吸湿性,应注意防潮。

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