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Merck

H1506

Sigma-Aldrich

3α-羟甾类脱氢酶 来源于睾丸酮假单胞菌

≥15 units/mg protein

别名:

3α-羟甾类:NAD(P)+氧化还原酶

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About This Item

CAS号:
EC號碼:
MDL號碼:
分類程式碼代碼:
12352204
NACRES:
NA.54

生物源

bacterial (Pseudomonas testosteroni)

品質等級

形狀

lyophilized solid

比活性

≥15 units/mg protein

純化經由

chromatography

成份

Protein, ≥25%

異物活動

β-hydroxysteroid dehydrogenase ≤0.5%
alcohol dehydrogenase ≤0.05%

儲存溫度

−20°C

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應用

来源于假单胞菌 睾丸激素的3α-羟基类固醇脱氢酶已被用于:
  • 制备复合电极平台,以用于测定雄甾酮
  • 磷酸钾缓冲液中,以用于血清中胆汁酸的生物发光测定
  • 通过酶法测定总胆汁盐

生化/生理作用

3α-羟基类固醇脱氢酶 (3α-HSD) 可以帮助调节雄激素靶组织中雄激素受体 (AR) 的占据。它也可阻碍雄激素的作用。

單位定義

在β-NAD+存在,pH8.9,25℃条件下,一个单位每分钟可氧化1.0 μmole雄甾酮。

外觀

含有磷酸钾缓冲盐、EDTA和DL-二硫苏糖醇的冻干粉末

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Bioluminescent assay for total bile acids in serum with use of bacterial luciferase.
Styrelius I, et al.
Clinical Chemistry, 29(6), 1123-1127 (1983)
Laurence Meyer et al.
Neurobiology of disease, 30(1), 30-41 (2008-02-23)
Identification of cellular targets pertinent for the development of effective therapies against pathological pain constitutes a difficult challenge. We combined several approaches to show that 3alpha-hydroxysteroid oxido-reductase (3alpha-HSOR), abundantly expressed in the spinal cord (SC), is a key target, the
Anne Z Steiner et al.
The Journal of clinical endocrinology and metabolism, 93(4), 1298-1303 (2008-02-07)
Dihydrotestosterone (DHT), the primary active androgen in peripheral target tissues, is metabolized by 3alpha-hydroxysteroid dehydrogenase type III (3alpha-HSD), encoded by the AKR1C2 gene, forming 5alpha-androstane-3alpha,17beta-diol (3alpha-diol). 3alpha-HSD may play a role in the pathogenesis of hirsutism. Our objective was to
Enzyme biosensor for androsterone based on 3alpha-hydroxysteroid dehydrogenase immobilized onto a carbon nanotubes/ionic liquid/NAD+ composite electrode
Mundaca RA, et al.
Talanta, 99(, 697-702 (2012)
Phytosterol ester processing in the small intestine; impact on cholesterol availability for absorption and chylomicron cholesterol incorporation in healthy humans
Amiot MJ, et al.
Journal of Lipid Research, jlr-M013730 (2011)

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