Merck
All Photos(2)

H1759

Sigma-Aldrich

(+)-生物素N-羟基琥珀酰亚胺酯

≥98% (HPLC), powder

Synonym(s):
(+)-生物素N-琥珀酰亚胺酯, d-生物素NHS酯, BNHS, N-琥珀酰亚胺基D-生物素, N-羟基琥珀酰亚胺生物素, NHS-生物素, 生物素-N-羟基琥珀酰亚胺
Empirical Formula (Hill Notation):
C14H19N3O5S
CAS Number:
Molecular Weight:
341.38
Beilstein:
4720781
MDL编号:
PubChem化学物质编号:
NACRES:
NA.46

质量水平

200

测定

≥98% (HPLC)

形式

powder

reaction suitability

reaction type: Biotinylations

颜色

white to off-white

mp

212 °C

溶解性

DMF: ≤50 mg/mL (Stable for at least one month in dry DMF)
DMSO: soluble

储存温度

−20°C

SMILES string

[H][C@]12CS[C@@H](CCCCC(=O)ON3C(=O)CCC3=O)[C@@]1([H])NC(=O)N2

InChI

1S/C14H19N3O5S/c18-10-5-6-11(19)17(10)22-12(20)4-2-1-3-9-13-8(7-23-9)15-14(21)16-13/h8-9,13H,1-7H2,(H2,15,16,21)/t8-,9-,13-/m0/s1

InChI key

YMXHPSHLTSZXKH-RVBZMBCESA-N

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应用

(+)-Biotin N-hydroxysuccinimide ester has been used:
  • to biotinylate Hyb 246-4 for surfactant protein D enzyme-linked immunosorbent assay (SP-D ELISA)[1]
  • to biotinylate proteins to form preformed complexes[2]
  • to label and modify monoclonal anti-microfibrillar-associated protein 4 (MFAP4) (HG-HYB 7-18) to bind to alphaLISA streptavidin-coated donor beads[3]

生化/生理作用

The N-hydroxysuccinimide ester (NHS)-biotin modification helps in the biotinylation of the target lysine residue at its ε-amino group and identifies the surface lysine residues. It is used as a tool to identify the interaction of proteins with several biological molecules, like protein-polysaccharide interactions, protein-protein interactions, protein-nucleic acid interactions, and protein-ligand interactions.[4] NHS-biotin may be used in the biotinylation of proteins and peptides. It is typically coupled to primary amine in the pH range of 6.5-8.5.

免责声明

除非在我们的产品目录或产品随附的其他公司文档中另有说明,我们的产品仅供研究使用并不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途、任何类型的食用或应用于人或动物。

储存分类代码

13 - Non Combustible Solids

WGK

WGK 3

闪点(F)

Not applicable

闪点(C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

Certificate of Analysis

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Certificate of Origin

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