H1759

(+)-生物素N-羟基琥珀酰亚胺酯

≥98% (HPLC), powder

• Synonym(s): (+)-生物素N-琥珀酰亚胺酯, d-生物素NHS酯, BNHS, N-琥珀酰亚胺基D-生物素, N-羟基琥珀酰亚胺生物素, NHS-生物素, 生物素-N-羟基琥珀酰亚胺
• Empirical Formula (Hill Notation): C₁₄H₁₉N₃O₅S
• CAS Number: 35013-72-0
• Molecular Weight: 341.38
• Beilstein: 4720781
• MDL编号: MFCD00078531
• PubChem化学物质编号: 24895479
• NACRES: NA.46

Properties

• 质量水平: 200
• 测定: ≥98% (HPLC)
• 形式: powder
• reaction suitability: reaction type: Biotinylations
• 颜色: white to off-white
• mp: 212 °C
• 溶解性: DMF: ≤50 mg/mL (Stable for at least one month in dry DMF); DMSO: soluble
• 储存温度: −20°C
• SMILES string: [H][C@]12CS[C@@H](CCCCC(=O)ON3C(=O)CCC3=O)[C@@]1([H])NC(=O)N2
• InChI: 1S/C14H19N3O5S/c18-10-5-6-11(19)17(10)22-12(20)4-2-1-3-9-13-8(7-23-9)15-14(21)16-13/h8-9,13H,1-7H2,(H2,15,16,21)/t8-,9-,13-/m0/s1
• InChI key: YMXHPSHLTSZXKH-RVBZMBCESA-N

Description

应用

(+)-Biotin N-hydroxysuccinimide ester has been used:

• to biotinylate Hyb 246-4 for surfactant protein D enzyme-linked immunosorbent assay (SP-D ELISA)[1]
• to biotinylate proteins to form preformed complexes[2]
• to label and modify monoclonal anti-microfibrillar-associated protein 4 (MFAP4) (HG-HYB 7-18) to bind to alphaLISA streptavidin-coated donor beads[3]

生化/生理作用

The N-hydroxysuccinimide ester (NHS)-biotin modification helps in the biotinylation of the target lysine residue at its ε-amino group and identifies the surface lysine residues. It is used as a tool to identify the interaction of proteins with several biological molecules, like protein-polysaccharide interactions, protein-protein interactions, protein-nucleic acid interactions, and protein-ligand interactions.[4] NHS-biotin may be used in the biotinylation of proteins and peptides. It is typically coupled to primary amine in the pH range of 6.5-8.5.

免责声明

除非在我们的产品目录或产品随附的其他公司文档中另有说明，我们的产品仅供研究使用并不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途、任何类型的食用或应用于人或动物。

Safety Information

• 储存分类代码: 13 - Non Combustible Solids
• WGK: WGK 3
• 闪点(F): Not applicable
• 闪点(C): Not applicable
• 个人防护装备: Eyeshields, Gloves, type N95 (US)