Merck

H1759

Sigma-Aldrich

(+)-生物素N-羟基琥珀酰亚胺酯

≥98% (HPLC), powder

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别名:
(+)-生物素N-琥珀酰亚胺酯, d-生物素NHS酯, BNHS, N-琥珀酰亚胺基D-生物素, N-羟基琥珀酰亚胺生物素, NHS-生物素, 生物素-N-羟基琥珀酰亚胺
Empirical Formula (Hill Notation):
C14H19N3O5S
CAS号:
分子量:
341.38
Beilstein:
4720781
MDL编号:
PubChem化学物质编号:
NACRES:
NA.46

检测方案

≥98% (HPLC)

形式

powder

reaction suitability

reaction type: Biotinylations

颜色

white to off-white

mp

212 °C

溶解性

DMF: ≤50 mg/mL (Stable for at least one month in dry DMF)
DMSO: soluble

储存温度

−20°C

SMILES string

[H][C@]12CS[C@@H](CCCCC(=O)ON3C(=O)CCC3=O)[C@@]1([H])NC(=O)N2

InChI

1S/C14H19N3O5S/c18-10-5-6-11(19)17(10)22-12(20)4-2-1-3-9-13-8(7-23-9)15-14(21)16-13/h8-9,13H,1-7H2,(H2,15,16,21)/t8-,9-,13-/m0/s1

InChI key

YMXHPSHLTSZXKH-RVBZMBCESA-N

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此商品
B2643B3295P1162
form

powder

form

powder

form

powder

form

powder

color

white to off-white

color

-

color

-

color

-

mp

212 °C

mp

-

mp

-

mp

-

solubility

DMF: ≤50 mg/mL (Stable for at least one month in dry DMF), DMSO: soluble

solubility

H2O: ≤2 mg/mL (with sonication), DMF: 50 mg/mL (Stable up to 2 months in dry DMF.)

solubility

DMF: 25 mg/mL

solubility

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

应用

(+)-生物素 N-羟基琥珀酰亚胺酯已用于:
  • 生物素化Hyb 246-4,用于表面活性剂蛋白 D 酶联免疫吸附试验(SP-D ELISA)
  • 生物素化蛋白质以形成预成型络合物
  • 标记和修饰单克隆抗微纤维相关蛋白 4 (MFAP4) (HG-HYB 7-18),以结合到alphaLISA 链霉亲和素涂层供体珠

生化/生理作用

N-羟基琥珀酰亚胺酯(NHS)生物素修饰有助于目标赖氨酸残基在ε-氨基上的生物素化,并识别表面赖氨酸残基。它被用作识别蛋白质与几种生物分子相互作用的工具,如蛋白质-多糖相互作用、蛋白质-蛋白质相互作用、蛋白质-核酸相互作用和蛋白质-配体相互作用。NHS-生物素可用于蛋白和肽的生物素化。它通常在6.5-8.5的pH范围内通常偶联至伯胺。

免责声明

除非在我们的产品目录或产品随附的其他公司文档中另有说明,我们的产品仅供研究使用并不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途、任何类型的食用或应用于人或动物。

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(F)

Not applicable

闪点(C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)


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