H3375

Sigma-Aldrich

羟乙基哌嗪乙硫磺酸

≥99.5% (titration)

别名:
4-(2-羟乙基)哌嗪-1-乙磺酸, N-(2-羟乙基)哌嗪-N′-(2-乙磺酸)
Empirical Formula (Hill Notation):
C8H18N2O4S
CAS号:
分子量:
238.30
Beilstein/REAXYS Number:
883043
EC 号:
MDL编号:
PubChem化学物质编号:
NACRES:
NA.25

质量水平

400

测定

≥99.5% (titration)

形式

crystalline powder

应用

cell culture | mammalian: suitable

pH值(酸碱度)

5.0-6.5 (25 °C, 238 g/L)

有效pH范围

6.8 - 8.2

pKa (25 °C)

7.5

溶解性

water: 500 mg/mL, clear, colorless

特色行业

Diagnostic Assay Manufacturing

SMILES string

OCCN1CCN(CC1)CCS(O)(=O)=O

InChI

1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)

InChI key

JKMHFZQWWAIEOD-UHFFFAOYSA-N

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一般描述

HEPES被视为可用于生物研究的最佳通用缓冲液之一。在生理pH下,该分子是两性离子的,并在pH 6.8至8.2下可有效作为缓冲剂。HEPES已用于一系列应用,包括组织培养。它常用于在空气中对细胞培养基进行缓冲。HEPES可用于Mg的体外实验。

应用

HEPES已用于:
  • 作为Danieau培养基中的一种成分,用于防止斑马鱼胚胎原肠胚形成后的黑色素形成
  • 作为Hank溶液中的一个组分用于铁的降解检测
  • 作为HEPES培养基中的一个组分用于细胞培养物的质粒转染
  • 与RPMI-1640、L-谷氨酰胺、FBS、丙酮酸钠和β-巯基乙醇用于大鼠胰岛瘤细胞的培养
  • 作为TCM199培养基中的一种补充剂用于核转移研究所需的新鲜卵母细胞洗涤

包装

5, 25, 50 kg in poly drum
25, 100, 250, 500 g in poly bottle
1 kg in poly bottle

其他说明

访问Redi-Dri产品列表 RDD002
利用 HEPES规格表,可轻松对比HEPES产品的规格。

法律信息

Redi-Dri is a trademark of Sigma-Aldrich Co. LLC

storage_class_code

13 - Non Combustible Solids

WGK Germany

WGK 1

闪点(F)

Not applicable

闪点(C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

分析证书

原产地证书 (CofO)

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What is the purpose of using HEPES in cell culture?

    HEPES provides supplemental buffering for media in pH range of 7.2 to 7.6. It has no nutritional value to cells.

  6. What is the concentration of HEPES used in cell culture?

    HEPES is typically used in cell culture in the range of 10-25 mM.

  7. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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