Merck

H3537

Sigma-Aldrich

羟乙基哌嗪乙硫磺酸 溶液

BioPerformance Certified, 1 M, suitable for cell culture, 0.2 μm filtered

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别名:
N-(2-羟乙基)哌嗪-N′-(2-乙磺酸)
CAS号:
MDL编号:
PubChem化学物质编号:
NACRES:
NA.25

等级

BioPerformance Certified

质量水平

无菌性

0.2 μm filtered

形式

liquid

浓度

1 M

technique(s)

cell culture | mammalian: suitable

杂质

Bioburden, tested
DNase, RNase, Protease, Nickase, free
endotoxin, tested

pH值(酸碱度)

5.0-6.0

useful pH range

6.8-8.2

痕量阳离子

Fe: <5 ppm
heavy metals (as Pb): <5 ppm

适用性

suitable for molecular biology

application(s)

diagnostic assay manufacturing

SMILES string

OCCN1CCN(CCS(=O)(O)=O)CC1

InChI

1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)

InChI key

JKMHFZQWWAIEOD-UHFFFAOYSA-N

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H0887H0887H7523
vibrant-m

H3537

羟乙基哌嗪乙硫磺酸 溶液

Premium Grade
vibrant-m

H0887

羟乙基哌嗪乙硫磺酸 溶液

Essential+ Grade
vibrant-m

H0887

羟乙基哌嗪乙硫磺酸 溶液

Essential+ Grade
vibrant-m

H7523

HEPES

Premium Grade
Quality Level

200

Quality Level

300

Quality Level

300

Quality Level

400

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

-

grade

BioPerformance Certified

grade

-

grade

-

grade

-

impurities

Bioburden, tested, endotoxin, tested, DNase, RNase, Protease, Nickase, free

impurities

endotoxin, tested

impurities

endotoxin, tested

impurities

Insoluble matter, passes filter test

form

liquid

form

liquid

form

liquid

form

powder

concentration

1 M

concentration

1 M

concentration

1 M

concentration

-

Quality Level

200

Quality Level

300

Quality Level

300

Quality Level

400

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

-

grade

BioPerformance Certified

grade

-

grade

-

grade

-

impurities

Bioburden, tested, endotoxin, tested, DNase, RNase, Protease, Nickase, free

impurities

endotoxin, tested

impurities

endotoxin, tested

impurities

Insoluble matter, passes filter test

form

liquid

form

liquid

form

liquid

form

powder

一般描述

HEPES被认为是生物研究中最好的通用缓冲液之一。在生物酸碱度下,分子是两性离子,在酸碱度为6.8至8.2时可有效地作为缓冲剂。HEPES已被广泛应用,包括组织培养。它通常用于缓冲空气中的细胞培养基。研究发现HEPES在体外镁实验中亦可应用。

应用

核糖核酸酶HEPES已被用于:
  • 补充Dulbecco“改良鹰”培养基以维持细胞系和RPMI培养基洗涤大鼠胰岛
  • 回收纯化的核糖核苷酸
  • 作为用于小核糖核酸分离和测序的HEPES/KOH缓冲液和腺苷酸化缓冲液的组分
  • 作为用于核提取物制备的缓冲液中的一种成分,还可作为RPMI-1640培养基的添加剂,用于胰岛的维持

储存分类代码

10 - Combustible liquids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable


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T1503
货号
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25G
包装规格/数量

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1000309185

输入内容 1.000309185)

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Timing of CRISPR/Cas9-related mRNA microinjection after activation as an important factor affecting genome editing efficiency in porcine oocytes.
Sato M, et al.
Theriogenology, 108, 29-38 (2018)
Expression of an uncleavable N-terminal RasGAP fragment in insulin-secreting cells increases their resistance toward apoptotic stimuli without affecting their glucose-induced insulin secretion.
Yang JY, et al.
The Journal of Biological Chemistry, 280(38), 32835-32842 (2005)
A Small RNA Isolation and Sequencing Protocol and Its Application to Assay CRISPR RNA Biogenesis in Bacteria.
Silas S, et al.
Bio-protocol, 8(4) (2018)
Masahiro Sato et al.
Theriogenology, 108, 29-38 (2017-12-02)
Recently, successful one-step genome editing by microinjection of CRISPR/Cas9-related mRNA components into the porcine zygote has been described. Given the relatively long gestational period and the high cost of housing swine, the establishment of an effective microinjection-based porcine genome editing
Masahiro Sato et al.
International journal of molecular sciences, 16(8), 17838-17856 (2015-08-08)
Some reports demonstrated successful genome editing in pigs by one-step zygote microinjection of mRNA of CRISPR/Cas9-related components. Given the relatively long gestation periods and the high cost of housing, the establishment of a single blastocyst-based assay for rapid optimization of

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