Merck

H3662

Sigma-Aldrich

HEPES 钠盐 溶液

1M, BioReagent, suitable for cell culture

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经验公式(希尔记法):
C8H17N2NaO4S
CAS号:
分子量:
260.29
MDL编号:
PubChem化学物质编号:
NACRES:
NA.25

质量水平

无菌性

sterile-filtered (0.2 μm filtered)

产品线

BioReagent

形式

solution

浓度

0.90-1.10 M

技术

cell culture | mammalian: suitable

杂质

Bioburden, tested
DNase, RNase. Protease, Nickase, free
endotoxin, tested
<5 ppm Heavy metals (as Pb)

痕量阳离子

Fe: <5 ppm

SMILES字符串

[Na+].OCCN1CCN(CC1)CCS([O-])(=O)=O

InChI

1S/C8H18N2O4S.Na/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14;/h11H,1-8H2,(H,12,13,14);/q;+1/p-1

InChI key

RDZTWEVXRGYCFV-UHFFFAOYSA-M

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此商品
H3784H7006H0887
HEPES 钠盐 溶液 1M, BioReagent, suitable for cell culture

H3662

HEPES 钠盐 溶液

Essential+ Grade
HEPES钠盐 钠盐 BioPerformance Certified, suitable for cell culture, &#8805;99.0%

H3784

HEPES钠盐 钠盐

Premium Grade
HEPES钠盐 钠盐 &#8805;99.0% (titration)

H7006

HEPES钠盐 钠盐

-
羟乙基哌嗪乙硫磺酸 溶液 1&#160;M, pH 7.0-7.6, sterile-filtered, BioReagent, suitable for cell culture

H0887

羟乙基哌嗪乙硫磺酸 溶液

Essential+ Grade
concentration

0.90-1.10 M

concentration

-

concentration

-

concentration

1 M

product line

BioReagent

product line

-

product line

-

product line

BioReagent, BioXtra

Quality Level

200

Quality Level

400

Quality Level

400

Quality Level

300

form

solution

form

powder

form

powder

form

liquid

sterility

sterile-filtered (0.2 μm filtered)

sterility

-

sterility

-

sterility

sterile-filtered

一般描述

HEPES是一种不结合镁、钙、锰(II)或铜(II)离子的通用型两性离子缓冲剂。在1966年,Norman Good博士及其同事将HEPES归类为Good′s缓冲液。它在生物pH下为两性离子型,并在pH 6.8至8.2的范围内最有效。

应用

HEPES钠盐溶液适合用于细胞培养研究。它可用于制备HEPES缓冲液。它可在通透性成纤维细胞的磷酸化检测过程中用作缓冲液。它可被用于组成复杂的透析缓冲液,用于通过放射免疫检测对血清中的游离甲状腺素进行测定。

储存分类代码

10 - Combustible liquids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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I J Chopra et al.
Thyroid : official journal of the American Thyroid Association, 6(4), 255-259 (1996-08-01)
We have devised a practical, sensitive, and reliable assay for measurement of free T3 concentration in serum. The assay employs a convenient and disposable plastic equilibrium dialysis cell and a buffer that resembles the in vivo biochemical environment (Nelson JC
Hydrogen ion buffers for biological research.
Good NE, et al.
BioChemistry: An Indian Journal, 5, 467-477 (1996)
C Estrada et al.
The Biochemical journal, 326 ( Pt 2), 369-376 (1997-09-18)
Although it has been demonstrated that NO inhibits the proliferation of different cell types, the mechanisms of its anti-mitotic action are not well understood. In this work we have studied the possible interaction of NO with the epidermal growth factor
J R Eidet et al.
Experimental eye research, 94(1), 109-116 (2011-12-14)
The use of amniotic membrane (AM) represents one of the major developments in ocular surface reconstruction. However, in a study on patients with primary pterygium, transplantation of AM with ex vivo expanded human conjunctival epithelial cells (HCjE) promoted earlier epithelialization
Qing Dai et al.
Analytical chemistry, 83(17), 6559-6564 (2011-08-02)
A ratiometric fluorescence sensor based on pyrene was designed for selective detection of heparin in HEPES (N-(2-hydroxyethyl)piperazine-N'-ethanesulfonic acid) buffer and serum sample. Pyrene and long-chain alkanes were linked through bisquaternary functionality in the sensor which could interact with heparin via

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