Merck

H6136

SAFC

Hanks平衡盐

Without sodium bicarbonate, powder, suitable for cell culture

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MDL编号:
NACRES:
NA.75

质量水平

形式

powder

technique(s)

cell culture | mammalian: suitable

储存温度

2-8°C

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technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

-

Quality Level

500

Quality Level

500

Quality Level

500

Quality Level

400

HBSS, salt solutions

HBSS, salt solutions

HBSS, salt solutions

HBSS, salt solutions

一般描述

平衡盐溶液(BSS)在细胞培养中至关重要,它作为一种灌溉、运输和稀释试剂,可维持细胞内和细胞外的渗透平衡。它为细胞提供水、无机离子和正常细胞代谢所需的能量。BSS还提供了一个缓冲系统,以维持培养基的最佳pH值范围(7.2-7.6)。

应用

Hanks平衡盐已用于:

  • 制备原液,用于培养小鼠原代肝细胞
  • 测定人成骨细胞培养中矿化结节的形成
  • 制备胚胎缓冲液以收集斑马鱼卵

数量

按照每升培养基9.8克粉末配制。

重悬

补充0.35 g/L碳酸氢钠。

其他说明

加入碳酸氢钠调节pH。

储存分类代码

11 - Combustible Solids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)


Certificates of Analysis (COA)

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25G
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实验方案

粉末培养基制备说明

粉末培养基及盐混合物极具吸湿性,应注意防潮。

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