Merck

H7006

Sigma-Aldrich

HEPES钠盐 钠盐

≥99.0% (titration)

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别名:
2-(4-(2-羟乙基)-1-哌嗪-1-基)乙磺酸钠, 4-(2-羟乙基)哌嗪-1-乙磺酸 钠盐, N-(2-羟乙基)哌嗪-N′-(2-乙磺酸) 钠盐
Empirical Formula (Hill Notation):
C8H17N2NaO4S
CAS号:
分子量:
260.29
EC 号:
MDL编号:
PubChem化学物质编号:
NACRES:
NA.25

质量水平

检测方案

≥99.0% (titration)

形式

powder

pH值(酸碱度)

10-12 (1 M in water)

useful pH range

6.8-8.2

pKa (25 °C)

7.5

溶解性

water: 0.1 M, clear, colorless

application(s)

diagnostic assay manufacturing

储存温度

room temp

SMILES string

[Na+].OCCN1CCN(CC1)CCS([O-])(=O)=O

InChI

1S/C8H18N2O4S.Na/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14;/h11H,1-8H2,(H,12,13,14);/q;+1/p-1

InChI key

RDZTWEVXRGYCFV-UHFFFAOYSA-M

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此商品
H3784RES6007H-A7H7637
HEPES sodium salt ≥99.0% (titration)

Sigma-Aldrich

H7006

HEPES钠盐 钠盐

-
HEPES sodium salt BioPerformance Certified, suitable for cell culture, ≥99.0%

Sigma-Aldrich

H3784

HEPES钠盐 钠盐

Premium Grade
HEPES sodium salt

SAFC

RES6007H-A7

HEPES钠盐 钠盐

-
form

powder

form

powder

form

powder

form

powder

pH

10-12 (1 M in water)

pH

10-12 (1 M in water)

pH

-

pH

7.4-7.7 (25 °C, 0.1 M in water)

solubility

water: 0.1 M, clear, colorless

solubility

water: 0.1 M, clear, colorless

solubility

-

solubility

water: soluble 0.5 g/mL

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing

application(s)

-

application(s)

diagnostic assay manufacturing

storage temp.

room temp

storage temp.

-

storage temp.

-

storage temp.

room temp

一般描述

HEPES盐,或称4-(2-羟乙基)哌嗪-1-乙烷磺酸钠盐,是一种两性离子生物缓冲液,广泛应用于生物学和生物化学研究。6.8-8.2 pH范围内的缓冲能力使其成为各种研究应用的理想选择。HEPES也是缓冲细胞培养基的常用试剂,其优点在于细胞毒性低于Tris 和磷酸盐等替代品。

HEPES被广泛应用于细胞生物学和生物化学研究,其多功能性在蛋白质纯化和提取过程中得到了充分体现,这一点是由于其在宽pH范围内的稳定性和最小的金属离子结合能力。 此外,HEPES 也是各种生物学和生物化学过程中的重要试剂,包括免疫沉淀、细胞裂解和活细胞成像,这使其成为各种研究应用中的多功能工具。

应用

HEPES缓冲液的应用包括:
  • 涉及金属离子的大多数研究
  • 涉及金属离子的研究中Tris 和磷酸盐的替代品
  • 植物研究中的研磨缓冲液
  • 凝胶电泳中的电泳缓冲液
  • 电穿孔研究中的缓冲液
  • Bradford或BCA 分析
  • 用于防止红细胞中蛋白质受损的提取缓冲液

它已被用于:
  • 低蔗糖缓冲液制备中的缓冲液成分
  • 改良Hanks′溶液(一种假性生理溶液)的缓冲液成分

特点和优势

  • 经测试证实重金属污染浓度低,从而确保其适用于各种应用
  • 易溶于水
  • 金属离子结合能力极低
  • 细胞毒性低于其它缓冲液如Tris和磷酸盐
  • 在宽pH范围内保持稳定

外形

冻干、喷雾干燥或结晶的材料。

其他说明

使用 HEPES 钠盐规格表可以非常方便地比较 HEPES 钠盐产品的规格。

储存分类代码

13 - Non Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)


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