Merck
All Photos(3)

H7523

Sigma-Aldrich

HEPES

BioXtra, pH 5.0-6.5 (1 M in H2O), ≥99.5% (titration)

Synonym(s):
4-(2-羟乙基)哌嗪-1-乙磺酸, N-(2-羟乙基)哌嗪-N′-(2-乙磺酸)
Empirical Formula (Hill Notation):
C8H18N2O4S
CAS Number:
Molecular Weight:
238.30
Beilstein:
883043
EC 号:
MDL编号:
PubChem化学物质编号:

产品线

BioXtra

质量水平

400

测定

≥99.5% (titration)

形式

powder

杂质

Insoluble matter, passes filter test

灼烧残渣 (900 °C)

≤0.1% (as SO4)

缺失

≤1.0% loss on drying, 110°C

pH值(酸碱度)

5.0-6.5 (1 M in H2O)

useful pH range

6.8-8.2

pKa (25 °C)

7.5

溶解性

H2O: 1 M, clear, colorless

痕量阴离子

chloride (Cl-): ≤0.05%

痕量阳离子

Al: ≤0.0005%
As: ≤0.0001%
Ba: ≤0.0005%
Bi: ≤0.0005%
Ca: ≤0.001%
Cd: ≤0.0005%
Co: ≤0.0005%
Cr: ≤0.0005%
Cu: ≤0.0005%
Fe: ≤0.0005%
K: ≤0.005%
Li: ≤0.0005%
Mg: ≤0.0005%
Mn: ≤0.0005%
Mo: ≤0.0005%
Na: ≤0.005%
Ni: ≤0.0005%
Pb: ≤0.0005%
Sr: ≤0.0005%
Zn: ≤0.0005%

吸光度

≤0.035 at 280 in H2O at 1 M
≤0.045 at 260 in H2O at 1 M

application(s)

diagnostic assay manufacturing

SMILES string

OCCN1CCN(CC1)CCS(O)(=O)=O

InChI

1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)

InChI key

JKMHFZQWWAIEOD-UHFFFAOYSA-N

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一般描述

HEPES被视为可用于生物研究的最佳通用缓冲液之一。在生理pH下,该分子是两性离子的,并在pH 6.8至8.2下可有效作为缓冲剂。HEPES已用于一系列应用,包括组织培养。它常用于在空气中对细胞培养基进行缓冲。HEPES 在 Mg 的体外实验中表现出了应用潜力。

应用

HEPES 已被用于:
  • 作为蛋白质样品制备中使用的均质化缓冲液的组分
  • 作为 PRP 制备的一部分,中和 PRP(富含血小板的血浆)溶液
  • 作为 2X PBS 缓冲液中的组分,其用于细胞系制备中的磷酸钙转染
  • 作为 RPMI 1640 培养基的补充剂,用于培养人脐静脉内皮细胞(HUVEC)和 M199 完全培养基,用于离体治疗睾丸组织。

包装

50, 250 g in poly bottle

其他说明

使用 HEPES 规格表可以非常方便地比较 HEPES 产品的规格。

储存分类代码

13 - Non Combustible Solids

WGK

WGK 1

闪点(F)

Not applicable

闪点(C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

Certificate of Analysis

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Certificate of Origin

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