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一般描述
D-β-羟丁酸脱氢酶 (BDH) 是一种与膜结合的脂质需求酶。
应用
β-来源于勒氏假单胞菌 的羟丁酸脱氢酶已被用于偶联检测以证实乙酰乙酸酯的形成。
适用于通过Williamson, D. H., and Mellanby, J., Methods of Enzymatic Analysis, Bergmeyer, H., ed., 2nd edition, 4, 1836 (1974)中介绍的方法测定乙酰乙酸酯和D(-)-3-羟基丁酸
生化/生理作用
在脱氮副球菌中，3-羟丁酸脱氢酶在胞内聚羟基丁酸酯和聚羟基戊酸酯的分解中起关键作用。
在哺乳动物系统中，β-羟丁酸脱氢酶位于线粒体内膜上，需要磷脂酰胆碱才能发挥活性。相反，在假单胞菌属中则为可溶性胞质酶，不需要磷脂别构活化剂。利用作为代谢能量源的酮体时，需要这种酶。其首先催化 3-羟基丁酸酯氧化成乙酰乙酸酯，这是酮体转化为柠檬酸的第一步，而后通过三羧酸循环（Krebs 循环）进一步参与代谢。
单位定义
在pH 7.8、温度37°C条件下，一单位每分钟可将1.0 μmol的 D-β-羟基丁酸氧化成乙酰乙酸盐。
外形
含有蔗糖、β-NAD和Tris缓冲盐的冻干粉末
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
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