所有图片(1)

I3390

Sigma-Aldrich

Iscove 改良杜氏培养基

liquid, sterile-filtered, With sodium bicarbonate, without L-glutamine, suitable for cell culture, suitable for hybridoma

别名:
IMDM
MDL编号:
NACRES:
NA.75

质量水平

400

无菌性

sterile-filtered

形式

liquid

technique(s)

cell culture | hybridoma: suitable
cell culture | mammalian: suitable

杂质

endotoxin, tested

组分

phenol red: yes
glucose: yes
L-glutamine: no

运输

ambient

储存温度

2-8°C

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应用

Guilbert和Iscove证明红细胞和巨噬细胞的前体细胞可以在完全确定的无血清培养基中进行培养,该培养基需补充白蛋白、转铁蛋白、卵磷脂和硒。该培养基是Dulbecco′s改良Eagle′s培养基(DME)的改良版本,其含有硒、额外的氨基酸和维生素、丙酮酸钠、HEPES缓冲液、以及硝酸钾(代替硝酸铁)。进一步的研究表明,Iscove′s培养基可以支持小鼠B淋巴细胞、骨髓造血组织、脂多糖刺激的B细胞、T淋巴细胞和各种杂交细胞的成长。

联系

需要FBS? 我们可以为您的血清计划提供协助。 请访问 血清计划 以获得更多信息。

重悬

以0.584 g/L补充 L-谷氨酰胺

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 1

闪点(F)

Not applicable

闪点(C)

Not applicable

分析证书

原产地证书 (CofO)

Dillon M Voss et al.
Cancer research, 80(21), 4681-4692 (2020-09-16)
Muscleblind-like proteins (MBNL) belong to a family of tissue-specific regulators of RNA metabolism that control premessenger RNA splicing. Inactivation of MBNL causes an adult-to-fetal alternative splicing transition, resulting in the development of myotonic dystrophy. We have previously shown that the
Sophie G Kellaway et al.
Haematologica, 106(6), 1569-1580 (2020-04-18)
Hematological malignancies are characterised by a block in differentiation, which in many cases is caused by recurrent mutations affecting the activity of hematopoietic transcription factors. RUNX1-EVI1 is a fusion protein formed by the t(3;21) translocation linking two transcription factors required
N N Iscove et al.
The Journal of experimental medicine, 147(3), 923-933 (1978-03-01)
Albumin, transferrin, and lipids can replace serum entirely for support of LPS-stimulated murine B lymphocytes in culture. In the presence of these compounds, growth and maturation to IgM and IgG secretion, induced by lipopolysaccharide (LPS), occurs at the same or
N Vriezen et al.
Biotechnology and bioengineering, 54(3), 272-286 (1997-05-05)
Glutamine is a major source of energy, carbon, and nitrogen for mammalian cells. The amount of glutamine present in commercial mammalian cell media is, however, not necessarily balanced with cell requirements. Therefore, the effects of glutamine limitation on the physiology
Kirsten Gillingwater
Parasitology, 145(7), 953-960 (2017-11-24)
Trypanosoma equiperdum is a protozoan parasite responsible for causing Dourine, a debilitating neglected veterinary disease, found worldwide affecting equids. It is the only pathogenic trypanosome species that does not require an invertebrate vector for transmission, thus being passed from animal

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