Merck

L1882

Sigma-Aldrich

来自 普通小麦 的凝集素(小麦)

agarose conjugate, saline suspension

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别名:
WGA, Wheat germ agglutinin
NACRES:
NA.32

偶联物

agarose conjugate

质量水平

形式

saline suspension

标记范围

5-10 mg per mL

基质

cross-linked 4% beaded agarose

基质活化

cyanogen bromide

容量

≥0.1 mg/mL binding capacity (ovomucoid)

储存温度

2-8°C

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此商品
L1394L5142L0636
form

saline suspension

form

saline suspension

form

lyophilized powder

form

lyophilized powder

matrix

cross-linked 4% beaded agarose

matrix

4% agarose

matrix

-

matrix

-

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

extent of labeling

5-10 mg per mL

extent of labeling

~6 mg per mL

extent of labeling

2-4 mol biotin per mol protein

extent of labeling

-

一般描述

凝集素是糖类结合蛋白,广泛存在于真菌、植物和动物中。凝集素的二级结构富含 β-链,表面具有糖类结合位点。

应用

用于纯化含有(D-N-乙酰氨基葡萄糖)2或N-乙酰神经氨酸的糖蛋白。

生化/生理作用

凝集素可用于鉴定、成像和靶向糖缀合物。与寡糖、糖蛋白和糖脂发生特异性相互作用。因此,凝集素用于微阵列测定,可实现有效的糖组谱分析。在植物和真菌内部,凝集素可抵御病原体或寄生物(feeder)侵袭。还参与宿主识别和组织粘附。
WGA 不具有血型特异性,但具有与 N-乙酰基-β-D-葡糖胺残基和 N-乙酰基-β-D-葡糖胺低聚物的亲和性。WGA 不含蛋白结合糖。

外形

悬浮于含15 mM叠氮化钠的1.0 M NaCl溶液中

制备说明

由亲和纯化凝集素制备偶联物。

储存分类代码

10 - Combustible liquids

WGK

WGK 3

个人防护装备

Eyeshields, Gloves


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K Paz et al.
The Journal of biological chemistry, 271(12), 6998-7003 (1996-03-22)
A structural analysis has been carried out to determine which part of the intracellular domain of the insulin receptor (IR) beta subunit is involved in direct interaction with the receptor substrates IRS-1 and Shc. Toward this end, the juxtamembrane (JM)
Binding sugars: from natural lectins to synthetic receptors and engineered neolectins
Arnaud J, et al.
Chemical Society Reviews, 42(11), 4798-4813 (2013)
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Cellular and molecular life sciences : CMLS, 79(11), 542-542 (2022-10-07)
Stem cells undergo cytokine-driven differentiation, but this process often takes longer than several weeks to complete. A novel mechanism for somatic stem cell differentiation via phagocytosing 'model cells' (apoptotic differentiated cells) was found to require only a short time frame.

商品

Find the right lectin for your research with our lectin selection guide, organized by lectin source/species, carbohydrate specificity, blood group specificity, and more.

This page shows how to solve practical problems that may occur when running an affinity chromatography column.

实验方案

This page shows how to purify glycoproteins and polysaccharides by affinity chromatography using Agarose Wheat Germ Lectin.

本页展示了在亲和色谱中如何在线性流率和体积流率之间换算。

This page shows how to convert between linear flow and volumetric flow rates in affinity chromatography.

该页面展示了如何使用GE医疗所提供的Tricorn™或XK色谱柱进行用于亲和层析的色谱柱填充和制备。

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